Create Intron Spanning Primers

This tool specifically tries to design Realtime-primers spanning introns. The resulting amplicons should only amplify cDNA sequences if the intron size is large enough and/or extenstion time of the PCR reaction is limited.

As above, all sequences are fetched and checked as above. Supported organisms are Human, Mouse, Rat and fruitfly.

A short summary is provided listing the primers, the amplicon and all targets (multiple targets are allowed if they are isoforms of the same gene).

Go to the Design page