Design Intron Spanning primers

This tool specifically tries to design Realtime-primers spanning introns. The resulting amplicons should only amplify cDNA sequences if the intron is large enough.

  • Target Information:
    • : RefSeq Gene Symbol
    • : Minimal Amplicon Size
    • : Maximal Amplicon Size
    • : Minimal intron size
  • Simulation Conditions:
    • : Natrium Concentration
    • : Magnesium Concentration
    • : Annealing Temperature
    • : Minimal dG for primer loops, 3'-dimers and amplicon folding at primer sites
    • : Minimal dG for primer homo- and heterodimer formation
    • : Minimal dG for overall amplicon folding (not at the primer binding sites
    • : Nr of Bases Considered as 3' for dimer simulation