qPCR PrimerDesign Pipeline
Created By Geert Vandeweyer
Design Results for BRAF
(Homo Sapiens, NCBI 37 / UCSC hg19)
| Settings Overview | |||
|---|---|---|---|
| Minimal Amplicon Size | 70 | Maximal Amplicon Size | 300 |
| Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
| Annealing Temperature | 60 °C | Minimal dG for primer folding | 0 |
| Minimal dG for primer dimer | -3 | Minimal dG for amplicon folding | -3 |
| Nr of bases considered as 3' | 5 | Minimal Intron Size | 3000 |
Sorting Options
Amplicon Details
Remark: Only top 100 primer pairs are shown)
| Exon 3 To Exon 4 (Pair 1) | |||
|---|---|---|---|
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 158 bpgenomic: 25,771 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 158 | 444 - 601 | |
| Exon 3 To Exon 5 (Pair 1) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 269 bpgenomic: 26,711 |
| Reverse (712,22) | AAGCCAGGAAATATCAGTGTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.787 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 269 | 444 - 712 | |
| Exon 3 To Exon 5 (Pair 2) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 295 bpgenomic: 26,737 |
| Reverse (737,22) | CTTCCACATGCAATTCTTCTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 295 | 443 - 737 | |
| Exon 3 To Exon 4 (Pair 2) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 159 bpgenomic: 25,772 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 159 | 442 - 600 | |
| Exon 3 To Exon 4 (Pair 3) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 157 bpgenomic: 25,770 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 157 | 443 - 599 | |
| Exon 3 To Exon 4 (Pair 4) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 195 bpgenomic: 25,808 |
| Reverse (636,22) | GGGATTAGACCTCTCATCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.586 kcal/mol | ||
| Forward-Reverse Dimers | -1.045 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 195 | 442 - 636 | |
| Exon 3 To Exon 5 (Pair 3) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 291 bpgenomic: 26,733 |
| Reverse (732,23) | ACATGCAATTCTTCTCCAGTAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 291 | 442 - 732 | |
| Exon 3 To Exon 4 (Pair 5) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 197 bpgenomic: 25,810 |
| Reverse (636,22) | GGGATTAGACCTCTCATCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.586 kcal/mol | ||
| Forward-Reverse Dimers | -1.045 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAATCCC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 197 | 440 - 636 | |
| Exon 3 To Exon 5 (Pair 4) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 289 bpgenomic: 26,731 |
| Reverse (732,23) | ACATGCAATTCTTCTCCAGTAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 289 | 444 - 732 | |
| Exon 3 To Exon 5 (Pair 5) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 273 bpgenomic: 26,715 |
| Reverse (715,23) | AGTAAGCCAGGAAATATCAGTGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.787 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 273 | 443 - 715 | |
| Exon 3 To Exon 5 (Pair 6) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 275 bpgenomic: 26,717 |
| Reverse (717,22) | CCAGTAAGCCAGGAAATATCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.601 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 275 | 443 - 717 | |
| Exon 3 To Exon 5 (Pair 7) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 299 bpgenomic: 26,741 |
| Reverse (741,23) | AACACTTCCACATGCAATTCTTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGTT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 299 | 443 - 741 | |
| Exon 3 To Exon 4 (Pair 6) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 196 bpgenomic: 25,809 |
| Reverse (632,22) | TTAGACCTCTCATCATCAGTGC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.167 kcal/mol | ||
| Forward-Reverse Dimers | -0.629 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAGGTCTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 196 | 437 - 632 | |
| Exon 3 To Exon 5 (Pair 8) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 294 bpgenomic: 26,736 |
| Reverse (732,23) | ACATGCAATTCTTCTCCAGTAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAA ACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 294 | 439 - 732 | |
| Exon 3 To Exon 4 (Pair 7) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 183 bpgenomic: 25,796 |
| Reverse (626,23) | CTCTCATCATCAGTGCTTTCTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 183 | 444 - 626 | |
| Exon 3 To Exon 4 (Pair 8) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 163 bpgenomic: 25,776 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 9) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 290 bpgenomic: 26,732 |
| Reverse (732,23) | ACATGCAATTCTTCTCCAGTAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 290 | 443 - 732 | |
| Exon 3 To Exon 4 (Pair 9) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 161 bpgenomic: 25,774 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 4 (Pair 10) | |||
| Forward (317,21) | ACCAGCAAGCTAGATGCACTC | Amplicon Size | cDNA: 286 bpgenomic: 25,899 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAAC GGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTCT TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 286 | 317 - 602 | |
| Exon 3 To Exon 5 (Pair 10) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 295 bpgenomic: 26,737 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAA ACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 295 | 439 - 733 | |
| Exon 3 To Exon 4 (Pair 11) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 186 bpgenomic: 25,799 |
| Reverse (624,23) | CTCATCATCAGTGCTTTCTTTAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.532 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 186 | 439 - 624 | |
| Exon 3 To Exon 4 (Pair 12) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 190 bpgenomic: 25,803 |
| Reverse (626,23) | CTCTCATCATCAGTGCTTTCTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.532 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 190 | 437 - 626 | |
| Exon 3 To Exon 4 (Pair 13) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 191 bpgenomic: 25,804 |
| Reverse (632,22) | TTAGACCTCTCATCATCAGTGC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.167 kcal/mol | ||
| Forward-Reverse Dimers | -0.629 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 191 | 442 - 632 | |
| Exon 3 To Exon 4 (Pair 14) | |||
| Forward (313,22) | ATACACCAGCAAGCTAGATGCA | Amplicon Size | cDNA: 290 bpgenomic: 25,903 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGG GAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTC CTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGT GGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAA ACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 290 | 313 - 602 | |
| Exon 3 To Exon 5 (Pair 11) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 297 bpgenomic: 26,739 |
| Reverse (740,23) | ACACTTCCACATGCAATTCTTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 297 | 444 - 740 | |
| Exon 3 To Exon 5 (Pair 12) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 276 bpgenomic: 26,718 |
| Reverse (719,22) | CTCCAGTAAGCCAGGAAATATC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.296 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 276 | 444 - 719 | |
| Exon 3 To Exon 5 (Pair 13) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 293 bpgenomic: 26,735 |
| Reverse (735,22) | TCCACATGCAATTCTTCTCCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 293 | 443 - 735 | |
| Exon 3 To Exon 4 (Pair 15) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 193 bpgenomic: 25,806 |
| Reverse (636,22) | GGGATTAGACCTCTCATCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.586 kcal/mol | ||
| Forward-Reverse Dimers | -1.045 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 193 | 444 - 636 | |
| Exon 3 To Exon 4 (Pair 16) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 193 bpgenomic: 25,806 |
| Reverse (632,22) | TTAGACCTCTCATCATCAGTGC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.167 kcal/mol | ||
| Forward-Reverse Dimers | -0.629 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 193 | 440 - 632 | |
| Exon 3 To Exon 5 (Pair 14) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 278 bpgenomic: 26,720 |
| Reverse (721,23) | TTCTCCAGTAAGCCAGGAAATAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.296 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 278 | 444 - 721 | |
| Exon 3 To Exon 4 (Pair 17) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 196 bpgenomic: 25,809 |
| Reverse (639,22) | TCTGGGATTAGACCTCTCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.603 kcal/mol | ||
| Forward-Reverse Dimers | -1.062 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 196 | 444 - 639 | |
| Exon 3 To Exon 4 (Pair 18) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 185 bpgenomic: 25,798 |
| Reverse (626,23) | CTCTCATCATCAGTGCTTTCTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 185 | 442 - 626 | |
| Exon 3 To Exon 4 (Pair 19) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 181 bpgenomic: 25,794 |
| Reverse (622,23) | CATCATCAGTGCTTTCTTTAGAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT G | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 181 | 442 - 622 | |
| Exon 3 To Exon 4 (Pair 20) | |||
| Forward (316,21) | CACCAGCAAGCTAGATGCACT | Amplicon Size | cDNA: 285 bpgenomic: 25,898 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAA CGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTC TTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGC ACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACA GAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 285 | 316 - 600 | |
| Exon 3 To Exon 4 (Pair 21) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 187 bpgenomic: 25,800 |
| Reverse (626,23) | CTCTCATCATCAGTGCTTTCTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 187 | 440 - 626 | |
| Exon 3 To Exon 4 (Pair 22) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 194 bpgenomic: 25,807 |
| Reverse (636,22) | GGGATTAGACCTCTCATCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.586 kcal/mol | ||
| Forward-Reverse Dimers | -1.045 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 194 | 443 - 636 | |
| Exon 3 To Exon 5 (Pair 15) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 296 bpgenomic: 26,738 |
| Reverse (735,22) | TCCACATGCAATTCTTCTCCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAA CCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 296 | 440 - 735 | |
| Exon 3 To Exon 5 (Pair 16) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 268 bpgenomic: 26,710 |
| Reverse (710,21) | GCCAGGAAATATCAGTGTCCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.721 kcal/mol in 3' area | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 268 | 443 - 710 | |
| Exon 3 To Exon 4 (Pair 23) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 179 bpgenomic: 25,792 |
| Reverse (622,23) | CATCATCAGTGCTTTCTTTAGAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 179 | 444 - 622 | |
| Exon 3 To Exon 4 (Pair 24) | |||
| Forward (316,21) | CACCAGCAAGCTAGATGCACT | Amplicon Size | cDNA: 286 bpgenomic: 25,899 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAA CGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTC TTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGC ACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACA GAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 286 | 316 - 601 | |
| Exon 3 To Exon 4 (Pair 25) | |||
| Forward (311,22) | GAATACACCAGCAAGCTAGATG | Amplicon Size | cDNA: 289 bpgenomic: 25,902 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.426 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | GAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTG GGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCT TCCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGAT GTGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAAC AAACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 289 | 311 - 599 | |
| Exon 3 To Exon 4 (Pair 26) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 195 bpgenomic: 25,808 |
| Reverse (637,22) | TGGGATTAGACCTCTCATCATC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.603 kcal/mol | ||
| Forward-Reverse Dimers | -1.062 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 195 | 443 - 637 | |
| Exon 3 To Exon 4 (Pair 27) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 162 bpgenomic: 25,775 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 162 | 440 - 601 | |
| Exon 3 To Exon 5 (Pair 17) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 290 bpgenomic: 26,732 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 290 | 444 - 733 | |
| Exon 3 To Exon 4 (Pair 28) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 195 bpgenomic: 25,808 |
| Reverse (638,22) | CTGGGATTAGACCTCTCATCAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.603 kcal/mol | ||
| Forward-Reverse Dimers | -1.062 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 195 | 444 - 638 | |
| Exon 3 To Exon 4 (Pair 29) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 188 bpgenomic: 25,801 |
| Reverse (624,23) | CTCATCATCAGTGCTTTCTTTAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol in 3' area | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.532 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 188 | 437 - 624 | |
| Exon 3 To Exon 4 (Pair 30) | |||
| Forward (317,21) | ACCAGCAAGCTAGATGCACTC | Amplicon Size | cDNA: 283 bpgenomic: 25,896 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAAC GGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTCT TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 283 | 317 - 599 | |
| Exon 3 To Exon 4 (Pair 31) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 163 bpgenomic: 25,776 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 18) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 291 bpgenomic: 26,733 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 291 | 444 - 734 | |
| Exon 3 To Exon 4 (Pair 32) | |||
| Forward (315,21) | ACACCAGCAAGCTAGATGCAC | Amplicon Size | cDNA: 287 bpgenomic: 25,900 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGA ACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCT CTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGG CACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAAC AGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 287 | 315 - 601 | |
| Exon 3 To Exon 5 (Pair 19) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 297 bpgenomic: 26,739 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAG AAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 297 | 437 - 733 | |
| Exon 3 To Exon 5 (Pair 20) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 298 bpgenomic: 26,740 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAG AAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 298 | 437 - 734 | |
| Exon 3 To Exon 4 (Pair 33) | |||
| Forward (312,22) | AATACACCAGCAAGCTAGATGC | Amplicon Size | cDNA: 290 bpgenomic: 25,903 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.008 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGG GGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTT CCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATG TGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACA AACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 290 | 312 - 601 | |
| Exon 3 To Exon 5 (Pair 21) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 297 bpgenomic: 26,739 |
| Reverse (735,22) | TCCACATGCAATTCTTCTCCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAA ACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 297 | 439 - 735 | |
| Exon 3 To Exon 4 (Pair 34) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 162 bpgenomic: 25,775 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 4 (Pair 35) | |||
| Forward (315,21) | ACACCAGCAAGCTAGATGCAC | Amplicon Size | cDNA: 285 bpgenomic: 25,898 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGA ACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCT CTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGG CACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAAC AGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 285 | 315 - 599 | |
| Exon 3 To Exon 5 (Pair 22) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 291 bpgenomic: 26,733 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 291 | 443 - 733 | |
| Exon 3 To Exon 4 (Pair 36) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 180 bpgenomic: 25,793 |
| Reverse (622,23) | CATCATCAGTGCTTTCTTTAGAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 180 | 443 - 622 | |
| Exon 3 To Exon 4 (Pair 37) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 160 bpgenomic: 25,773 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 160 | 443 - 602 | |
| Exon 3 To Exon 5 (Pair 23) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 279 bpgenomic: 26,721 |
| Reverse (721,23) | TTCTCCAGTAAGCCAGGAAATAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.296 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 279 | 443 - 721 | |
| Exon 3 To Exon 5 (Pair 24) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 301 bpgenomic: 26,743 |
| Reverse (737,22) | CTTCCACATGCAATTCTTCTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAG AAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAA G | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 301 | 437 - 737 | |
| Exon 3 To Exon 5 (Pair 25) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 296 bpgenomic: 26,738 |
| Reverse (737,22) | CTTCCACATGCAATTCTTCTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 296 | 442 - 737 | |
| Exon 3 To Exon 4 (Pair 38) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 159 bpgenomic: 25,772 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 159 | 443 - 601 | |
| Exon 3 To Exon 4 (Pair 39) | |||
| Forward (311,22) | GAATACACCAGCAAGCTAGATG | Amplicon Size | cDNA: 292 bpgenomic: 25,905 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.426 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | GAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTG GGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCT TCCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGAT GTGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAAC AAACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 292 | 311 - 602 | |
| Exon 3 To Exon 5 (Pair 26) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 269 bpgenomic: 26,711 |
| Reverse (710,21) | GCCAGGAAATATCAGTGTCCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.721 kcal/mol in 3' area | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 269 | 442 - 710 | |
| Exon 3 To Exon 4 (Pair 40) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 184 bpgenomic: 25,797 |
| Reverse (622,23) | CATCATCAGTGCTTTCTTTAGAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.532 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 184 | 439 - 622 | |
| Exon 3 To Exon 4 (Pair 41) | |||
| Forward (317,21) | ACCAGCAAGCTAGATGCACTC | Amplicon Size | cDNA: 285 bpgenomic: 25,898 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAAC GGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTCT TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 285 | 317 - 601 | |
| Exon 3 To Exon 5 (Pair 27) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 298 bpgenomic: 26,740 |
| Reverse (740,23) | ACACTTCCACATGCAATTCTTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 298 | 443 - 740 | |
| Exon 3 To Exon 5 (Pair 28) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 293 bpgenomic: 26,735 |
| Reverse (732,23) | ACATGCAATTCTTCTCCAGTAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAA CCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 293 | 440 - 732 | |
| Exon 3 To Exon 4 (Pair 42) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 194 bpgenomic: 25,807 |
| Reverse (632,22) | TTAGACCTCTCATCATCAGTGC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.167 kcal/mol | ||
| Forward-Reverse Dimers | -0.629 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 194 | 439 - 632 | |
| Exon 3 To Exon 5 (Pair 29) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 300 bpgenomic: 26,742 |
| Reverse (741,23) | AACACTTCCACATGCAATTCTTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGTT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 300 | 442 - 741 | |
| Exon 3 To Exon 4 (Pair 43) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 196 bpgenomic: 25,809 |
| Reverse (638,22) | CTGGGATTAGACCTCTCATCAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.603 kcal/mol | ||
| Forward-Reverse Dimers | -1.062 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 196 | 443 - 638 | |
| Exon 3 To Exon 5 (Pair 30) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 294 bpgenomic: 26,736 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAA CCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 294 | 440 - 733 | |
| Exon 3 To Exon 4 (Pair 44) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 156 bpgenomic: 25,769 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 156 | 444 - 599 | |
| Exon 3 To Exon 5 (Pair 31) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 294 bpgenomic: 26,736 |
| Reverse (735,22) | TCCACATGCAATTCTTCTCCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 294 | 442 - 735 | |
| Exon 3 To Exon 4 (Pair 45) | |||
| Forward (316,21) | CACCAGCAAGCTAGATGCACT | Amplicon Size | cDNA: 284 bpgenomic: 25,897 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAA CGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTC TTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGC ACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACA GAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 284 | 316 - 599 | |
| Exon 3 To Exon 5 (Pair 32) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 299 bpgenomic: 26,741 |
| Reverse (737,22) | CTTCCACATGCAATTCTTCTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAA ACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 299 | 439 - 737 | |
| Exon 3 To Exon 4 (Pair 46) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 158 bpgenomic: 25,771 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 158 | 443 - 600 | |
| Exon 3 To Exon 5 (Pair 33) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 292 bpgenomic: 26,734 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 292 | 443 - 734 | |
| Exon 3 To Exon 4 (Pair 47) | |||
| Forward (317,21) | ACCAGCAAGCTAGATGCACTC | Amplicon Size | cDNA: 284 bpgenomic: 25,897 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGAAC GGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCTCT TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 284 | 317 - 600 | |
| Exon 3 To Exon 4 (Pair 48) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 161 bpgenomic: 25,774 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 161 | 440 - 600 | |
| Exon 3 To Exon 4 (Pair 49) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 160 bpgenomic: 25,773 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 160 | 442 - 601 | |
| Exon 3 To Exon 4 (Pair 50) | |||
| Forward (311,22) | GAATACACCAGCAAGCTAGATG | Amplicon Size | cDNA: 290 bpgenomic: 25,903 |
| Reverse (600,21) | CTGTCTCGGACTGTAACTCCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.426 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | GAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTG GGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCT TCCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGAT GTGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAAC AAACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 290 | 311 - 600 | |
| Exon 3 To Exon 4 (Pair 51) | |||
| Forward (311,22) | GAATACACCAGCAAGCTAGATG | Amplicon Size | cDNA: 291 bpgenomic: 25,904 |
| Reverse (601,21) | ACTGTCTCGGACTGTAACTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.426 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.173 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | GAATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTG GGGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCT TCCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGAT GTGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAAC AAACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 291 | 311 - 601 | |
| Exon 3 To Exon 5 (Pair 34) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 271 bpgenomic: 26,713 |
| Reverse (712,22) | AAGCCAGGAAATATCAGTGTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.787 kcal/mol | ||
| Reverse Dimers | -1.364 kcal/mol | ||
| Forward-Reverse Dimers | -2.445 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 271 | 442 - 712 | |
| Exon 3 To Exon 5 (Pair 35) | |||
| Forward (443,22) | CTTTCAGTGCTACCTTCATCTC | Amplicon Size | cDNA: 300 bpgenomic: 26,742 |
| Reverse (742,23) | CAACACTTCCACATGCAATTCTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol in 3' area | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGC AACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACA GTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATG AGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCA ATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGTTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 300 | 443 - 742 | |
| Exon 3 To Exon 4 (Pair 52) | |||
| Forward (312,22) | AATACACCAGCAAGCTAGATGC | Amplicon Size | cDNA: 291 bpgenomic: 25,904 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.008 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AATACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGG GGAACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTT CCTCTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATG TGGCACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACA AACAGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 291 | 312 - 602 | |
| Exon 3 To Exon 4 (Pair 53) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 166 bpgenomic: 25,779 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 166 | 437 - 602 | |
| Exon 3 To Exon 5 (Pair 36) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 299 bpgenomic: 26,741 |
| Reverse (735,22) | TCCACATGCAATTCTTCTCCAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTG ATGATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAG AAACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 299 | 437 - 735 | |
| Exon 3 To Exon 4 (Pair 54) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 194 bpgenomic: 25,807 |
| Reverse (637,22) | TGGGATTAGACCTCTCATCATC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.53 kcal/mol | ||
| Reverse Dimers | -0.603 kcal/mol | ||
| Forward-Reverse Dimers | -1.062 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 194 | 444 - 637 | |
| Exon 3 To Exon 5 (Pair 37) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 292 bpgenomic: 26,734 |
| Reverse (733,23) | CACATGCAATTCTTCTCCAGTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 292 | 442 - 733 | |
| Exon 3 To Exon 4 (Pair 55) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 160 bpgenomic: 25,773 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 160 | 440 - 599 | |
| Exon 3 To Exon 5 (Pair 38) | |||
| Forward (444,23) | TTTCAGTGCTACCTTCATCTCTT | Amplicon Size | cDNA: 294 bpgenomic: 26,736 |
| Reverse (737,22) | CTTCCACATGCAATTCTTCTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAGCA ACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGACAG TGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGATGA GAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACCAA TTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 294 | 444 - 737 | |
| Exon 3 To Exon 5 (Pair 39) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 301 bpgenomic: 26,743 |
| Reverse (742,23) | CAACACTTCCACATGCAATTCTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol in 3' area | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -2.269 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGGAAGTGTT G | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 301 | 442 - 742 | |
| Exon 3 To Exon 4 (Pair 56) | |||
| Forward (315,21) | ACACCAGCAAGCTAGATGCAC | Amplicon Size | cDNA: 288 bpgenomic: 25,901 |
| Reverse (602,21) | GACTGTCTCGGACTGTAACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.586 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 0.61 kcal/mol | ||
| Reverse Dimers | -1.004 kcal/mol | ||
| Forward-Reverse Dimers | -1.072 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | ACACCAGCAAGCTAGATGCACTCCAACAAAGAGAACAACAGTTATTGGAATCTCTGGGGA ACGGAACTGATTTTTCTGTTTCTAGCTCTGCATCAATGGATACCGTTACATCTTCTTCCT CTTCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGG CACGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAAC AGAGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 288 | 315 - 602 | |
| Exon 3 To Exon 5 (Pair 40) | |||
| Forward (442,22) | CCTTTCAGTGCTACCTTCATCT | Amplicon Size | cDNA: 293 bpgenomic: 26,735 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.996 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | CCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGGAG CAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGGAC AGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATGAT GAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAACC AATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 293 | 442 - 734 | |
| Exon 3 To Exon 5 (Pair 41) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 295 bpgenomic: 26,737 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAAA CCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 295 | 440 - 734 | |
| Exon 3 To Exon 4 (Pair 57) | |||
| Forward (437,22) | TCTAGCCTTTCAGTGCTACCTT | Amplicon Size | cDNA: 163 bpgenomic: 25,776 |
| Reverse (599,21) | TGTCTCGGACTGTAACTCCAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.613 kcal/mol | ||
| Reverse Dimers | -0.87 kcal/mol | ||
| Forward-Reverse Dimers | -1.83 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCA CGGAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAG AGGACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 4 (Pair 58) | |||
| Forward (440,22) | AGCCTTTCAGTGCTACCTTCAT | Amplicon Size | cDNA: 183 bpgenomic: 25,796 |
| Reverse (622,23) | CATCATCAGTGCTTTCTTTAGAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.104 kcal/mol | |
| Forward Dimers | -0.833 kcal/mol | ||
| Reverse Loops | 1.941 kcal/mol | ||
| Reverse Dimers | -0.959 kcal/mol | ||
| Forward-Reverse Dimers | -1.355 kcal/mol | ||
| Amplicon Folding | -1.455 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACGG AGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAGG ACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGATG ATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 183 | 440 - 622 | |
| Exon 3 To Exon 5 (Pair 42) | |||
| Forward (439,22) | TAGCCTTTCAGTGCTACCTTCA | Amplicon Size | cDNA: 296 bpgenomic: 26,738 |
| Reverse (734,22) | CCACATGCAATTCTTCTCCAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.874 kcal/mol | |
| Forward Dimers | -1.01 kcal/mol | ||
| Reverse Loops | 1.195 kcal/mol | ||
| Reverse Dimers | -1.814 kcal/mol | ||
| Forward-Reverse Dimers | -1.03 kcal/mol | ||
| Amplicon Folding | -2.511 kcal/mol outside primersite | ||
| Amplicon Sequence | TAGCCTTTCAGTGCTACCTTCATCTCTTTCAGTTTTTCAAAATCCCACAGATGTGGCACG GAGCAACCCCAAGTCACCACAAAAACCTATCGTTAGAGTCTTCCTGCCCAACAAACAGAG GACAGTGGTACCTGCAAGGTGTGGAGTTACAGTCCGAGACAGTCTAAAGAAAGCACTGAT GATGAGAGGTCTAATCCCAGAGTGCTGTGCTGTTTACAGAATTCAGGATGGAGAGAAGAA ACCAATTGGTTGGGACACTGATATTTCCTGGCTTACTGGAGAAGAATTGCATGTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_004333 (BRAF) | 296 | 439 - 734 | |
See below for some more details.
Program Output:
Loading ...