qPCR PrimerDesign Pipeline
Created By Geert Vandeweyer
Design Results for FYN
(Homo Sapiens, NCBI 37 / UCSC hg19)
| Settings Overview | |||
|---|---|---|---|
| Minimal Amplicon Size | 70 | Maximal Amplicon Size | 300 |
| Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
| Annealing Temperature | 60 °C | Minimal dG for primer folding | 0 |
| Minimal dG for primer dimer | -3 | Minimal dG for amplicon folding | -3 |
| Nr of bases considered as 3' | 5 | Minimal Intron Size | 3000 |
Sorting Options
Amplicon Details
Remark: Only top 100 primer pairs are shown)
| Exon 3 To Exon 5 (Pair 1) | |||
|---|---|---|---|
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 151 bpgenomic: 60,562 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 2) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 163 bpgenomic: 60,574 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 71 | 559 - 629 | |
| Exon 3 To Exon 5 (Pair 3) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 153 bpgenomic: 60,564 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 4) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 152 bpgenomic: 60,563 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 5) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 150 bpgenomic: 60,561 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 6) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 160 bpgenomic: 60,571 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 68 | 562 - 629 | |
| Exon 3 To Exon 5 (Pair 7) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 155 bpgenomic: 60,566 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 8) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 148 bpgenomic: 60,559 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 10) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 163 bpgenomic: 60,574 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 71 | 558 - 628 | |
| Exon 3 To Exon 5 (Pair 11) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 152 bpgenomic: 60,563 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 12) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 151 bpgenomic: 60,562 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 14) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 158 bpgenomic: 60,569 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 66 | 559 - 624 | |
| Exon 3 To Exon 5 (Pair 15) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 147 bpgenomic: 60,558 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 16) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 159 bpgenomic: 60,570 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 67 | 559 - 625 | |
| Exon 3 To Exon 5 (Pair 17) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 154 bpgenomic: 60,565 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 18) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 149 bpgenomic: 60,560 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 19) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 152 bpgenomic: 60,563 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 20) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 159 bpgenomic: 60,570 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 67 | 563 - 629 | |
| Exon 3 To Exon 5 (Pair 21) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 165 bpgenomic: 60,576 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 73 | 557 - 629 | |
| Exon 3 To Exon 5 (Pair 22) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 164 bpgenomic: 60,575 |
| Reverse (694,21) | TCCTTACATTGCACACAGCCC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 72 | 559 - 630 | |
| Exon 3 To Exon 5 (Pair 23) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 161 bpgenomic: 60,572 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 69 | 557 - 625 | |
| Exon 3 To Exon 5 (Pair 24) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 166 bpgenomic: 60,577 |
| Reverse (694,21) | TCCTTACATTGCACACAGCCC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 74 | 557 - 630 | |
| Exon 3 To Exon 5 (Pair 25) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 157 bpgenomic: 60,568 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 65 | 565 - 629 | |
| Exon 3 To Exon 4 (Pair 5) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 120 bpgenomic: 21,554 |
| Reverse (658,21) | TTCACGTAAGCCTCCCTTCTC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 1.04 kcal/mol in 3' area | ||
| Reverse Dimers | -2.764 kcal/mol | ||
| Forward-Reverse Dimers | -1.713 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 26) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 155 bpgenomic: 60,566 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 27) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 164 bpgenomic: 60,575 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 72 | 557 - 628 | |
| Exon 3 To Exon 5 (Pair 28) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 155 bpgenomic: 60,566 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 29) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 154 bpgenomic: 60,565 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 30) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 162 bpgenomic: 60,573 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 70 | 559 - 628 | |
| Exon 3 To Exon 5 (Pair 31) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 157 bpgenomic: 60,568 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 32) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 165 bpgenomic: 60,576 |
| Reverse (694,21) | TCCTTACATTGCACACAGCCC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 73 | 558 - 630 | |
| Exon 3 To Exon 4 (Pair 15) | |||
| Forward (500,21) | ACCAGTCCTGCCTCTGTTGTA | Amplicon Size | cDNA: 97 bpgenomic: 21,531 |
| Reverse (596,23) | TTGGCTTATACCCAACACTTGAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.825 kcal/mol | |
| Forward Dimers | 0.136 kcal/mol | ||
| Reverse Loops | 1.043 kcal/mol in 3' area | ||
| Reverse Dimers | -0.676 kcal/mol | ||
| Forward-Reverse Dimers | -1.729 kcal/mol | ||
| Amplicon Folding | 0.22 kcal/mol outside primersite | ||
| Amplicon Sequence | ACCAGTCCTGCCTCTGTTGTAGAAGACATGTGGTGTATATAAAGTTTGTGATCGTTGGCG GACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 33) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 150 bpgenomic: 60,561 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 34) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 159 bpgenomic: 60,570 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 67 | 558 - 624 | |
| Exon 3 To Exon 5 (Pair 35) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 162 bpgenomic: 60,573 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 70 | 558 - 627 | |
| Exon 3 To Exon 5 (Pair 36) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 153 bpgenomic: 60,564 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 37) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 155 bpgenomic: 60,566 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 63 | 567 - 629 | |
| Exon 3 To Exon 5 (Pair 38) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 149 bpgenomic: 60,560 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 39) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 158 bpgenomic: 60,569 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 40) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 155 bpgenomic: 60,566 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 41) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 153 bpgenomic: 60,564 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA GGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 43) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 158 bpgenomic: 60,569 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 66 | 563 - 628 | |
| Exon 3 To Exon 5 (Pair 44) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 156 bpgenomic: 60,567 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 45) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 156 bpgenomic: 60,567 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 46) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 156 bpgenomic: 60,567 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 47) | |||
| Forward (537,23) | TATAAAGTTTGTGATCGTTGGCG | Amplicon Size | cDNA: 151 bpgenomic: 60,562 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.32 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA GGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTG AAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 4 (Pair 21) | |||
| Forward (499,20) | GACCAGTCCTGCCTCTGTTG | Amplicon Size | cDNA: 98 bpgenomic: 21,532 |
| Reverse (596,23) | TTGGCTTATACCCAACACTTGAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.825 kcal/mol | |
| Forward Dimers | 0.042 kcal/mol | ||
| Reverse Loops | 1.043 kcal/mol in 3' area | ||
| Reverse Dimers | -0.676 kcal/mol | ||
| Forward-Reverse Dimers | -1.729 kcal/mol | ||
| Amplicon Folding | 0.22 kcal/mol outside primersite | ||
| Amplicon Sequence | GACCAGTCCTGCCTCTGTTGTAGAAGACATGTGGTGTATATAAAGTTTGTGATCGTTGGC GGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 4 (Pair 22) | |||
| Forward (539,22) | TAAAGTTTGTGATCGTTGGCGG | Amplicon Size | cDNA: 121 bpgenomic: 21,555 |
| Reverse (659,21) | CTTCACGTAAGCCTCCCTTCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.158 kcal/mol in 3' area | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 1.04 kcal/mol in 3' area | ||
| Reverse Dimers | -2.764 kcal/mol | ||
| Forward-Reverse Dimers | -1.713 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCCAAGG AGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAA G | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 48) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 160 bpgenomic: 60,571 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 68 | 557 - 624 | |
| Exon 3 To Exon 5 (Pair 49) | |||
| Forward (529,24) | GTGGTGTATATAAAGTTTGTGATC | Amplicon Size | cDNA: 163 bpgenomic: 60,574 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTA TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 71 | 557 - 627 | |
| Exon 3 To Exon 5 (Pair 50) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 157 bpgenomic: 60,568 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 51) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 161 bpgenomic: 60,572 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 69 | 559 - 627 | |
| Exon 3 To Exon 5 (Pair 52) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 159 bpgenomic: 60,570 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 67 | 562 - 628 | |
| Exon 3 To Exon 5 (Pair 53) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 164 bpgenomic: 60,575 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 72 | 558 - 629 | |
| Exon 3 To Exon 5 (Pair 54) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 154 bpgenomic: 60,565 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 55) | |||
| Forward (535,24) | TATATAAAGTTTGTGATCGTTGGC | Amplicon Size | cDNA: 157 bpgenomic: 60,568 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGCC AAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTACG TGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 56) | |||
| Forward (531,24) | GGTGTATATAAAGTTTGTGATCGT | Amplicon Size | cDNA: 156 bpgenomic: 60,567 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATA AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 57) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 158 bpgenomic: 60,569 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 3 To Exon 5 (Pair 58) | |||
| Forward (530,24) | TGGTGTATATAAAGTTTGTGATCG | Amplicon Size | cDNA: 160 bpgenomic: 60,571 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.293 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGTGTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTAT AAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGC TTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002037 (FYN) | 68 | 558 - 625 | |
| Exon 3 To Exon 5 (Pair 59) | |||
| Forward (534,24) | GTATATAAAGTTTGTGATCGTTGG | Amplicon Size | cDNA: 153 bpgenomic: 60,564 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.214 kcal/mol | |
| Forward Dimers | -0.404 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.092 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTATATAAAGTTTGTGATCGTTGGCGGACATTTTGAAATGATCAAGTGTTGGGTATAAGC CAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCTTAC GTGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| () | - | ||
| Exon 4 To Exon 5 (Pair 1) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 106 bpgenomic: 39,083 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 106 | 19 - 124 | |
| Exon 4 To Exon 5 (Pair 2) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 120 bpgenomic: 39,097 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 120 | 5 - 124 | |
| Exon 4 To Exon 5 (Pair 3) | |||
| Forward (581,21) | GTTGGGTATAAGCCAAGGAGC | Amplicon Size | cDNA: 109 bpgenomic: 39,086 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.905 kcal/mol | |
| Forward Dimers | -0.764 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.284 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAG AAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 109 | 13 - 121 | |
| Exon 4 To Exon 5 (Pair 4) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 116 bpgenomic: 39,093 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 116 | 5 - 120 | |
| Exon 4 To Exon 5 (Pair 5) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 100 bpgenomic: 39,077 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 100 | 19 - 118 | |
| Exon 4 To Exon 5 (Pair 6) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 123 bpgenomic: 39,100 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTA AGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 123 | 3 - 125 | |
| Exon 4 To Exon 5 (Pair 7) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 122 bpgenomic: 39,099 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTA AG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 122 | 3 - 124 | |
| Exon 4 To Exon 5 (Pair 8) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 122 bpgenomic: 39,099 |
| Reverse (694,21) | TCCTTACATTGCACACAGCCC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG GA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 122 | 5 - 126 | |
| Exon 4 To Exon 5 (Pair 9) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 103 bpgenomic: 39,080 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 103 | 21 - 123 | |
| Exon 4 To Exon 5 (Pair 10) | |||
| Forward (591,20) | AGCCAAGGAGCTGAGAGAGG | Amplicon Size | cDNA: 95 bpgenomic: 39,072 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.72 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 95 | 23 - 117 | |
| Exon 4 To Exon 5 (Pair 11) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 113 bpgenomic: 39,090 |
| Reverse (699,23) | CTTTATCCTTACATTGCACACAG | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 1.829 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.628 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 113 | 19 - 131 | |
| Exon 4 To Exon 5 (Pair 12) | |||
| Forward (628,20) | CTGAGGAGCTGAGAAGGGAG | Amplicon Size | cDNA: 72 bpgenomic: 39,049 |
| Reverse (699,23) | CTTTATCCTTACATTGCACACAG | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.866 kcal/mol in 3' area | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 1.829 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.335 kcal/mol | ||
| Amplicon Folding | 0.422 kcal/mol inside primersite | ||
| Amplicon Sequence | CTGAGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT GTAAGGATAAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 72 | 60 - 131 | |
| Exon 4 To Exon 5 (Pair 13) | |||
| Forward (591,20) | AGCCAAGGAGCTGAGAGAGG | Amplicon Size | cDNA: 97 bpgenomic: 39,074 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.72 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 97 | 23 - 119 | |
| Exon 4 To Exon 5 (Pair 14) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 111 bpgenomic: 39,088 |
| Reverse (699,23) | CTTTATCCTTACATTGCACACAG | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 1.829 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.628 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 111 | 21 - 131 | |
| Exon 4 To Exon 5 (Pair 15) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 124 bpgenomic: 39,101 |
| Reverse (694,21) | TCCTTACATTGCACACAGCCC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTA AGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 124 | 3 - 126 | |
| Exon 4 To Exon 5 (Pair 16) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 99 bpgenomic: 39,076 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 99 | 19 - 117 | |
| Exon 4 To Exon 5 (Pair 17) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 121 bpgenomic: 39,098 |
| Reverse (693,21) | CCTTACATTGCACACAGCCCA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG G | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 121 | 5 - 125 | |
| Exon 4 To Exon 5 (Pair 18) | |||
| Forward (591,20) | AGCCAAGGAGCTGAGAGAGG | Amplicon Size | cDNA: 109 bpgenomic: 39,086 |
| Reverse (699,23) | CTTTATCCTTACATTGCACACAG | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.72 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 1.829 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.628 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAGGATAAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 109 | 23 - 131 | |
| Exon 4 To Exon 5 (Pair 19) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 117 bpgenomic: 39,094 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 117 | 5 - 121 | |
| Exon 4 To Exon 5 (Pair 20) | |||
| Forward (581,21) | GTTGGGTATAAGCCAAGGAGC | Amplicon Size | cDNA: 108 bpgenomic: 39,085 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.905 kcal/mol | |
| Forward Dimers | -0.764 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.284 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAG AAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 108 | 13 - 120 | |
| Exon 4 To Exon 5 (Pair 21) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 100 bpgenomic: 39,077 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 100 | 21 - 120 | |
| Exon 4 To Exon 5 (Pair 22) | |||
| Forward (591,20) | AGCCAAGGAGCTGAGAGAGG | Amplicon Size | cDNA: 102 bpgenomic: 39,079 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.72 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 102 | 23 - 124 | |
| Exon 4 To Exon 5 (Pair 23) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 121 bpgenomic: 39,098 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTA A | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 121 | 3 - 123 | |
| Exon 4 To Exon 5 (Pair 24) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 103 bpgenomic: 39,080 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 103 | 19 - 121 | |
| Exon 4 To Exon 5 (Pair 25) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 119 bpgenomic: 39,096 |
| Reverse (691,23) | TTACATTGCACACAGCCCATTAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 119 | 5 - 123 | |
| Exon 4 To Exon 5 (Pair 26) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 101 bpgenomic: 39,078 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 101 | 19 - 119 | |
| Exon 4 To Exon 5 (Pair 27) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 101 bpgenomic: 39,078 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 101 | 21 - 121 | |
| Exon 4 To Exon 5 (Pair 28) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 104 bpgenomic: 39,081 |
| Reverse (692,22) | CTTACATTGCACACAGCCCATT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGTAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 104 | 21 - 124 | |
| Exon 4 To Exon 5 (Pair 29) | |||
| Forward (581,21) | GTTGGGTATAAGCCAAGGAGC | Amplicon Size | cDNA: 105 bpgenomic: 39,082 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.905 kcal/mol | |
| Forward Dimers | -0.764 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.284 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAG AAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 105 | 13 - 117 | |
| Exon 4 To Exon 5 (Pair 30) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 99 bpgenomic: 39,076 |
| Reverse (687,23) | ATTGCACACAGCCCATTATCTAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAAT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 99 | 21 - 119 | |
| Exon 4 To Exon 5 (Pair 31) | |||
| Forward (591,20) | AGCCAAGGAGCTGAGAGAGG | Amplicon Size | cDNA: 98 bpgenomic: 39,075 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.72 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGGCT TACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 98 | 23 - 120 | |
| Exon 4 To Exon 5 (Pair 32) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 116 bpgenomic: 39,093 |
| Reverse (686,23) | TTGCACACAGCCCATTATCTAAA | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 116 | 3 - 118 | |
| Exon 4 To Exon 5 (Pair 33) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 118 bpgenomic: 39,095 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 118 | 3 - 120 | |
| Exon 4 To Exon 5 (Pair 34) | |||
| Forward (571,23) | ATGATCAAGTGTTGGGTATAAGC | Amplicon Size | cDNA: 119 bpgenomic: 39,096 |
| Reverse (689,22) | ACATTGCACACAGCCCATTATC | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTG AGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 119 | 3 - 121 | |
| Exon 4 To Exon 5 (Pair 35) | |||
| Forward (569,24) | AAATGATCAAGTGTTGGGTATAAG | Amplicon Size | cDNA: 117 bpgenomic: 39,094 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AAATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTC TGAGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 117 | 1 - 117 | |
| Exon 4 To Exon 5 (Pair 36) | |||
| Forward (589,21) | TAAGCCAAGGAGCTGAGAGAG | Amplicon Size | cDNA: 97 bpgenomic: 39,074 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGAGG CTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 97 | 21 - 117 | |
| Exon 4 To Exon 5 (Pair 37) | |||
| Forward (587,22) | TATAAGCCAAGGAGCTGAGAGA | Amplicon Size | cDNA: 102 bpgenomic: 39,079 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.179 kcal/mol | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.983 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAGGAGCTGAGAAGGGA GGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 102 | 19 - 120 | |
| Exon 4 To Exon 5 (Pair 38) | |||
| Forward (569,24) | AAATGATCAAGTGTTGGGTATAAG | Amplicon Size | cDNA: 120 bpgenomic: 39,097 |
| Reverse (688,22) | CATTGCACACAGCCCATTATCT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.583 kcal/mol | |
| Forward Dimers | -2.036 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | AAATGATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTC TGAGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCAATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 120 | 1 - 120 | |
| Exon 4 To Exon 5 (Pair 39) | |||
| Forward (626,20) | GTCTGAGGAGCTGAGAAGGG | Amplicon Size | cDNA: 74 bpgenomic: 39,051 |
| Reverse (699,23) | CTTTATCCTTACATTGCACACAG | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.866 kcal/mol in 3' area | |
| Forward Dimers | -2.732 kcal/mol | ||
| Reverse Loops | 1.829 kcal/mol in 3' area | ||
| Reverse Dimers | -1.266 kcal/mol | ||
| Forward-Reverse Dimers | -2.335 kcal/mol | ||
| Amplicon Folding | 0.422 kcal/mol inside primersite | ||
| Amplicon Sequence | GTCTGAGGAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA ATGTAAGGATAAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 74 | 58 - 131 | |
| Exon 4 To Exon 5 (Pair 40) | |||
| Forward (573,22) | GATCAAGTGTTGGGTATAAGCC | Amplicon Size | cDNA: 113 bpgenomic: 39,090 |
| Reverse (685,23) | TGCACACAGCCCATTATCTAAAT | Nr. of Isoforms | 1 out of 3 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.327 kcal/mol in 3' area | |
| Forward Dimers | -0.18 kcal/mol | ||
| Reverse Loops | 0.653 kcal/mol | ||
| Reverse Dimers | -1.124 kcal/mol | ||
| Forward-Reverse Dimers | -2.392 kcal/mol | ||
| Amplicon Folding | -1.359 kcal/mol outside primersite | ||
| Amplicon Sequence | GATCAAGTGTTGGGTATAAGCCAAGGAGCTGAGAGAGGGGGAGACCAGCGCAGGTCTGAG GAGCTGAGAAGGGAGGCTTACGTGAAGGGAATTTAGATAATGGGCTGTGTGCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_153048 (FYN) | 113 | 5 - 117 | |
See below for some more details.
Program Output:
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