qPCR PrimerDesign Pipeline
Created By Geert Vandeweyer
Design Results for HMG20A
(Homo Sapiens, NCBI 37 / UCSC hg19)
| Settings Overview | |||
|---|---|---|---|
| Minimal Amplicon Size | 70 | Maximal Amplicon Size | 300 |
| Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
| Annealing Temperature | 60 °C | Minimal dG for primer folding | 0 |
| Minimal dG for primer dimer | -3 | Minimal dG for amplicon folding | -3 |
| Nr of bases considered as 3' | 5 | Minimal Intron Size | 3000 |
Sorting Options
Amplicon Details
Remark: Only top 100 primer pairs are shown)
| Exon 2 To Exon 3 (Pair 1) | |||
|---|---|---|---|
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 145 bpgenomic: 10,737 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 146 | 137 - 282 | |
| Exon 2 To Exon 3 (Pair 2) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 144 bpgenomic: 10,736 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 150 | 138 - 287 | |
| Exon 2 To Exon 3 (Pair 3) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 141 bpgenomic: 10,733 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 143 | 146 - 288 | |
| Exon 2 To Exon 3 (Pair 4) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 181 bpgenomic: 10,773 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACCAC T | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 151 - 289 | |
| Exon 2 To Exon 3 (Pair 5) | |||
| Forward (152,22) | GCTACACATCACTTGACACCAT | Amplicon Size | cDNA: 139 bpgenomic: 10,731 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -0.002 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.353 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAAC TTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGT AATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 137 | 151 - 287 | |
| Exon 2 To Exon 3 (Pair 6) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 140 bpgenomic: 10,732 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 177 | 106 - 282 | |
| Exon 2 To Exon 3 (Pair 7) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 122 bpgenomic: 10,714 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 144 | 144 - 287 | |
| Exon 2 To Exon 3 (Pair 8) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 144 bpgenomic: 10,736 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 120 | 146 - 265 | |
| Exon 2 To Exon 3 (Pair 9) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 138 bpgenomic: 10,730 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 138 | 149 - 286 | |
| Exon 2 To Exon 3 (Pair 10) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 182 bpgenomic: 10,774 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACCAC TG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 182 | 108 - 289 | |
| Exon 2 To Exon 3 (Pair 11) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 144 bpgenomic: 10,736 |
| Reverse (281,22) | CCAGATCATTACTCTCCTTGGA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.23 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -0.873 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 148 | 137 - 284 | |
| Exon 2 To Exon 3 (Pair 12) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 149 bpgenomic: 10,741 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 181 | 108 - 288 | |
| Exon 2 To Exon 3 (Pair 13) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 132 bpgenomic: 10,724 |
| Reverse (281,22) | CCAGATCATTACTCTCCTTGGA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.23 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.105 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 134 | 149 - 282 | |
| Exon 2 To Exon 3 (Pair 14) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 182 bpgenomic: 10,774 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACC AC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 180 | 108 - 287 | |
| Exon 2 To Exon 3 (Pair 15) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 181 bpgenomic: 10,773 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACC A | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 175 | 106 - 280 | |
| Exon 2 To Exon 3 (Pair 16) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 122 bpgenomic: 10,714 |
| Reverse (230,21) | GGGTGGAGCTAGTCATCAAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -0.94 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 124 | 106 - 229 | |
| Exon 2 To Exon 3 (Pair 17) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 146 bpgenomic: 10,738 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 140 | 143 - 282 | |
| Exon 2 To Exon 3 (Pair 18) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 127 bpgenomic: 10,719 |
| Reverse (233,21) | GTAGGGTGGAGCTAGTCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol in 3' area | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.099 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 135 | 150 - 284 | |
| Exon 2 To Exon 3 (Pair 19) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 153 bpgenomic: 10,745 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 146 | 144 - 289 | |
| Exon 2 To Exon 3 (Pair 20) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 126 bpgenomic: 10,718 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 158 | 108 - 265 | |
| Exon 2 To Exon 3 (Pair 21) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 142 bpgenomic: 10,734 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 137 | 146 - 282 | |
| Exon 2 To Exon 3 (Pair 22) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 179 bpgenomic: 10,771 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 182 | 108 - 289 | |
| Exon 2 To Exon 3 (Pair 23) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 125 bpgenomic: 10,717 |
| Reverse (231,21) | AGGGTGGAGCTAGTCATCAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.099 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 125 | 108 - 232 | |
| Exon 2 To Exon 3 (Pair 24) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 143 bpgenomic: 10,735 |
| Reverse (281,22) | CCAGATCATTACTCTCCTTGGA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.23 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -0.873 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 132 | 149 - 280 | |
| Exon 2 To Exon 3 (Pair 25) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 143 bpgenomic: 10,735 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 181 | 106 - 286 | |
| Exon 2 To Exon 3 (Pair 26) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 120 bpgenomic: 10,712 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 146 - 284 | |
| Exon 2 To Exon 3 (Pair 27) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 145 bpgenomic: 10,737 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 150 - 288 | |
| Exon 2 To Exon 3 (Pair 28) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 141 bpgenomic: 10,733 |
| Reverse (281,22) | CCAGATCATTACTCTCCTTGGA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.23 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -0.873 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 137 | 144 - 280 | |
| Exon 2 To Exon 3 (Pair 29) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 152 bpgenomic: 10,744 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 143 | 146 - 288 | |
| Exon 2 To Exon 3 (Pair 30) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 143 bpgenomic: 10,735 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 153 | 137 - 289 | |
| Exon 2 To Exon 3 (Pair 31) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 138 bpgenomic: 10,730 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 127 | 106 - 232 | |
| Exon 2 To Exon 3 (Pair 32) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 125 bpgenomic: 10,717 |
| Reverse (233,21) | GTAGGGTGGAGCTAGTCATCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol in 3' area | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -0.94 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 180 | 108 - 287 | |
| Exon 2 To Exon 3 (Pair 33) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 184 bpgenomic: 10,776 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACC ACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 183 | 106 - 288 | |
| Exon 2 To Exon 3 (Pair 34) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 144 bpgenomic: 10,736 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 142 | 143 - 284 | |
| Exon 2 To Exon 3 (Pair 35) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 148 bpgenomic: 10,740 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 149 | 140 - 288 | |
| Exon 2 To Exon 3 (Pair 36) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 150 bpgenomic: 10,742 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 144 | 137 - 280 | |
| Exon 2 To Exon 3 (Pair 37) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 134 bpgenomic: 10,726 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 134 | 149 - 282 | |
| Exon 2 To Exon 3 (Pair 38) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 139 bpgenomic: 10,731 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 133 | 150 - 282 | |
| Exon 2 To Exon 3 (Pair 39) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 147 bpgenomic: 10,739 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 179 | 106 - 284 | |
| Exon 2 To Exon 3 (Pair 40) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 149 bpgenomic: 10,741 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 179 | 108 - 286 | |
| Exon 2 To Exon 3 (Pair 41) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 147 bpgenomic: 10,739 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 144 | 146 - 289 | |
| Exon 2 To Exon 3 (Pair 42) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 142 bpgenomic: 10,734 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 144 | 143 - 286 | |
| Exon 2 To Exon 3 (Pair 43) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 147 bpgenomic: 10,739 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 138 | 149 - 286 | |
| Exon 2 To Exon 3 (Pair 44) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 123 bpgenomic: 10,715 |
| Reverse (231,21) | AGGGTGGAGCTAGTCATCAAG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -0.94 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 180 | 108 - 287 | |
| Exon 2 To Exon 3 (Pair 45) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 142 bpgenomic: 10,734 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 127 | 108 - 234 | |
| Exon 2 To Exon 3 (Pair 46) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 140 bpgenomic: 10,732 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 116 | 150 - 265 | |
| Exon 2 To Exon 3 (Pair 47) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 141 bpgenomic: 10,733 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 146 | 144 - 289 | |
| Exon 2 To Exon 3 (Pair 48) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 137 bpgenomic: 10,729 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 135 | 150 - 284 | |
| Exon 2 To Exon 3 (Pair 49) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 150 bpgenomic: 10,742 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 150 | 137 - 286 | |
| Exon 2 To Exon 3 (Pair 50) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 136 bpgenomic: 10,728 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 120 | 146 - 265 | |
| Exon 2 To Exon 3 (Pair 51) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 146 bpgenomic: 10,738 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 136 | 151 - 286 | |
| Exon 2 To Exon 3 (Pair 52) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 151 bpgenomic: 10,743 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 181 | 108 - 288 | |
| Exon 2 To Exon 3 (Pair 53) | |||
| Forward (152,22) | GCTACACATCACTTGACACCAT | Amplicon Size | cDNA: 134 bpgenomic: 10,726 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -0.002 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.767 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAAC TTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGT AATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 183 | 106 - 288 | |
| Exon 2 To Exon 3 (Pair 54) | |||
| Forward (152,22) | GCTACACATCACTTGACACCAT | Amplicon Size | cDNA: 132 bpgenomic: 10,724 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -0.002 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.105 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAAC TTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGT AATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 136 | 149 - 284 | |
| Exon 2 To Exon 3 (Pair 55) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 125 bpgenomic: 10,717 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 149 | 141 - 289 | |
| Exon 2 To Exon 3 (Pair 56) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 183 bpgenomic: 10,775 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACC ACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 160 | 106 - 265 | |
| Exon 2 To Exon 3 (Pair 57) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 146 bpgenomic: 10,738 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 151 - 289 | |
| Exon 2 To Exon 3 (Pair 58) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 146 bpgenomic: 10,738 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 123 | 143 - 265 | |
| Exon 2 To Exon 3 (Pair 59) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 151 bpgenomic: 10,743 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 182 | 106 - 287 | |
| Exon 2 To Exon 3 (Pair 60) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 141 bpgenomic: 10,733 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 149 - 287 | |
| Exon 2 To Exon 3 (Pair 61) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 119 bpgenomic: 10,711 |
| Reverse (265,21) | CTTGGAACCGTCTTCATCTGC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 135 | 146 - 280 | |
| Exon 2 To Exon 3 (Pair 62) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 147 bpgenomic: 10,739 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 150 | 140 - 289 | |
| Exon 2 To Exon 3 (Pair 63) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 139 bpgenomic: 10,731 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 138 | 150 - 287 | |
| Exon 2 To Exon 3 (Pair 64) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 129 bpgenomic: 10,721 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 123 | 143 - 265 | |
| Exon 2 To Exon 3 (Pair 65) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 133 bpgenomic: 10,725 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 123 | 108 - 230 | |
| Exon 2 To Exon 3 (Pair 66) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 137 bpgenomic: 10,729 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 138 | 149 - 286 | |
| Exon 2 To Exon 3 (Pair 67) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 149 bpgenomic: 10,741 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 146 | 144 - 289 | |
| Exon 2 To Exon 3 (Pair 68) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 124 bpgenomic: 10,716 |
| Reverse (230,21) | GGGTGGAGCTAGTCATCAAGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.099 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 179 | 106 - 284 | |
| Exon 2 To Exon 3 (Pair 69) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 145 bpgenomic: 10,737 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 180 | 108 - 287 | |
| Exon 2 To Exon 3 (Pair 70) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 129 bpgenomic: 10,721 |
| Reverse (235,20) | CGGTAGGGTGGAGCTAGTCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol in 3' area | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.099 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACCCTACCG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 119 | 146 - 264 | |
| Exon 2 To Exon 3 (Pair 71) | |||
| Forward (107,22) | GTCTCTACAAGGAAGATAACGG | Amplicon Size | cDNA: 123 bpgenomic: 10,715 |
| Reverse (229,22) | GGTGGAGCTAGTCATCAAGTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.965 kcal/mol | |
| Forward Dimers | -0.479 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.099 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GTCTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTG ACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCC ACC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 184 | 106 - 289 | |
| Exon 2 To Exon 3 (Pair 72) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 144 bpgenomic: 10,736 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 143 | 144 - 286 | |
| Exon 2 To Exon 3 (Pair 73) | |||
| Forward (144,23) | AAAGCAAAGCTACACATCACTTG | Amplicon Size | cDNA: 123 bpgenomic: 10,715 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGA TGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCA AGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 120 | 146 - 265 | |
| Exon 2 To Exon 3 (Pair 74) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 145 bpgenomic: 10,737 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 142 | 143 - 284 | |
| Exon 2 To Exon 3 (Pair 75) | |||
| Forward (145,23) | AAGCAAAGCTACACATCACTTGA | Amplicon Size | cDNA: 139 bpgenomic: 10,731 |
| Reverse (283,22) | AGCCAGATCATTACTCTCCTTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.296 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGAT GGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAA GGAGAGTAATGATCTGGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 133 | 150 - 282 | |
| Exon 2 To Exon 3 (Pair 76) | |||
| Forward (141,24) | TATAAAGCAAAGCTACACATCACT | Amplicon Size | cDNA: 148 bpgenomic: 10,740 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAG AGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTT CCAAGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 151 | 138 - 288 | |
| Exon 2 To Exon 3 (Pair 77) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 152 bpgenomic: 10,744 |
| Reverse (290,22) | CAGTGGTAGCCAGATCATTACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGGAGAGTAATGATCTGGCTACCACTG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 146 | 144 - 289 | |
| Exon 2 To Exon 3 (Pair 78) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 127 bpgenomic: 10,719 |
| Reverse (235,20) | CGGTAGGGTGGAGCTAGTCA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol in 3' area | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -0.94 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTACCG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 175 | 108 - 282 | |
| Exon 2 To Exon 3 (Pair 79) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 121 bpgenomic: 10,713 |
| Reverse (229,22) | GGTGGAGCTAGTCATCAAGTTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.754 kcal/mol | ||
| Reverse Dimers | -2.822 kcal/mol | ||
| Forward-Reverse Dimers | -0.94 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC C | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 181 | 108 - 288 | |
| Exon 2 To Exon 3 (Pair 80) | |||
| Forward (138,24) | TTCTATAAAGCAAAGCTACACATC | Amplicon Size | cDNA: 150 bpgenomic: 10,742 |
| Reverse (287,22) | TGGTAGCCAGATCATTACTCTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TTCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAA GAGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACG GTTCCAAGGAGAGTAATGATCTGGCTACCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 129 | 137 - 265 | |
| Exon 2 To Exon 3 (Pair 81) | |||
| Forward (139,24) | TCTATAAAGCAAAGCTACACATCA | Amplicon Size | cDNA: 128 bpgenomic: 10,720 |
| Reverse (266,21) | CCTTGGAACCGTCTTCATCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.464 kcal/mol | ||
| Reverse Dimers | -0.668 kcal/mol | ||
| Forward-Reverse Dimers | -0.783 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | TCTATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAG AGAGATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGG TTCCAAGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 116 | 150 - 265 | |
| Exon 2 To Exon 3 (Pair 82) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 139 bpgenomic: 10,731 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 136 | 149 - 284 | |
| Exon 2 To Exon 3 (Pair 83) | |||
| Forward (150,22) | AAGCTACACATCACTTGACACC | Amplicon Size | cDNA: 140 bpgenomic: 10,732 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAA ACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGA GTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 181 | 106 - 286 | |
| Exon 2 To Exon 3 (Pair 84) | |||
| Forward (151,22) | AGCTACACATCACTTGACACCA | Amplicon Size | cDNA: 135 bpgenomic: 10,727 |
| Reverse (285,22) | GTAGCCAGATCATTACTCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol | |
| Forward Dimers | -1.696 kcal/mol | ||
| Reverse Loops | 1.913 kcal/mol in 3' area | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.094 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | AGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAA CTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAG TAATGATCTGGCTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 141 | 149 - 289 | |
| Exon 2 To Exon 3 (Pair 85) | |||
| Forward (109,22) | CTCTACAAGGAAGATAACGGAC | Amplicon Size | cDNA: 180 bpgenomic: 10,772 |
| Reverse (288,22) | GTGGTAGCCAGATCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.014 kcal/mol | |
| Forward Dimers | -0.412 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -1.183 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | CTCTACAAGGAAGATAACGGACTGTAAAATTCTATAAAGCAAAGCTACACATCACTTGAC ACCATACACCATCTTGGTTACATAATGAAGAGAGATGGAAAACTTGATGACTAGCTCCAC CCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGGAGAGTAATGATCTGGCTACCAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 132 | 149 - 280 | |
| Exon 2 To Exon 3 (Pair 86) | |||
| Forward (147,22) | GCAAAGCTACACATCACTTGAC | Amplicon Size | cDNA: 143 bpgenomic: 10,735 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.055 kcal/mol in 3' area | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGAGATGG AAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTCCAAGG AGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 143 | 146 - 288 | |
| Exon 2 To Exon 3 (Pair 87) | |||
| Forward (142,24) | ATAAAGCAAAGCTACACATCACTT | Amplicon Size | cDNA: 148 bpgenomic: 10,740 |
| Reverse (289,22) | AGTGGTAGCCAGATCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.278 kcal/mol | |
| Forward Dimers | -2.778 kcal/mol | ||
| Reverse Loops | 1.427 kcal/mol | ||
| Reverse Dimers | -0.806 kcal/mol | ||
| Forward-Reverse Dimers | -2.385 kcal/mol | ||
| Amplicon Folding | -0.542 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAAGCAAAGCTACACATCACTTGACACCATACACCATCTTGGTTACATAATGAAGAGA GATGGAAAACTTGATGACTAGCTCCACCCTACCGCCCCTTTTTGCAGATGAAGACGGTTC CAAGGAGAGTAATGATCTGGCTACCACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 139 | 150 - 288 | |
| Exon 4 To Exon 6 (Pair 1) | |||
| Forward (401,22) | AATGCAGCAGAAGGCAATGAAC | Amplicon Size | cDNA: 301 bpgenomic: 6,610 |
| Reverse (701,22) | GTTCCTTCATGTAACGCTCCTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.458 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | AATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGT TGGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCC CTTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCA GAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCT GAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAAGGAA C | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 301 | 400 - 700 | |
| Exon 4 To Exon 6 (Pair 2) | |||
| Forward (398,22) | TCAAATGCAGCAGAAGGCAATG | Amplicon Size | cDNA: 295 bpgenomic: 6,604 |
| Reverse (692,22) | TGTAACGCTCCTTATCTCTGTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol in 3' area | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.042 kcal/mol in 3' area | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | TCAAATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGA GGTTGGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCC CCCCTTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGA CCAGAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCT CCTGAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 295 | 397 - 691 | |
| Exon 4 To Exon 6 (Pair 3) | |||
| Forward (402,22) | ATGCAGCAGAAGGCAATGAACA | Amplicon Size | cDNA: 299 bpgenomic: 6,608 |
| Reverse (700,23) | TTCCTTCATGTAACGCTCCTTAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.458 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTT GGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCC TTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAG AAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTG AGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAAGGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 297 | 403 - 699 | |
| Exon 4 To Exon 6 (Pair 4) | |||
| Forward (404,21) | GCAGCAGAAGGCAATGAACAG | Amplicon Size | cDNA: 299 bpgenomic: 6,608 |
| Reverse (702,22) | AGTTCCTTCATGTAACGCTCCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -0.584 kcal/mol | ||
| Reverse Loops | 2.302 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.458 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTTGG TCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCCTT ACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAGAA GTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTGAG GAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAAGGAACT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 293 | 403 - 695 | |
| Exon 4 To Exon 6 (Pair 5) | |||
| Forward (402,22) | ATGCAGCAGAAGGCAATGAACA | Amplicon Size | cDNA: 300 bpgenomic: 6,609 |
| Reverse (701,22) | GTTCCTTCATGTAACGCTCCTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.458 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTT GGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCC TTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAG AAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTG AGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAAGGAAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 299 | 401 - 699 | |
| Exon 4 To Exon 6 (Pair 6) | |||
| Forward (402,22) | ATGCAGCAGAAGGCAATGAACA | Amplicon Size | cDNA: 290 bpgenomic: 6,599 |
| Reverse (691,22) | GTAACGCTCCTTATCTCTGTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.042 kcal/mol in 3' area | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTT GGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCC TTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAG AAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTG AGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 300 | 401 - 700 | |
| Exon 4 To Exon 6 (Pair 7) | |||
| Forward (404,21) | GCAGCAGAAGGCAATGAACAG | Amplicon Size | cDNA: 293 bpgenomic: 6,602 |
| Reverse (696,23) | TTCATGTAACGCTCCTTATCTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -0.584 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTTGG TCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCCTT ACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAGAA GTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTGAG GAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 288 | 403 - 690 | |
| Exon 4 To Exon 6 (Pair 8) | |||
| Forward (401,22) | AATGCAGCAGAAGGCAATGAAC | Amplicon Size | cDNA: 296 bpgenomic: 6,605 |
| Reverse (696,23) | TTCATGTAACGCTCCTTATCTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | AATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGT TGGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCC CTTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCA GAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCT GAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 298 | 398 - 695 | |
| Exon 4 To Exon 6 (Pair 9) | |||
| Forward (402,22) | ATGCAGCAGAAGGCAATGAACA | Amplicon Size | cDNA: 293 bpgenomic: 6,602 |
| Reverse (694,22) | CATGTAACGCTCCTTATCTCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTT GGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCC TTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAG AAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTG AGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 292 | 400 - 691 | |
| Exon 4 To Exon 6 (Pair 10) | |||
| Forward (401,22) | AATGCAGCAGAAGGCAATGAAC | Amplicon Size | cDNA: 290 bpgenomic: 6,599 |
| Reverse (690,22) | TAACGCTCCTTATCTCTGTCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.042 kcal/mol in 3' area | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | AATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGT TGGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCC CTTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCA GAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCT GAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 297 | 397 - 693 | |
| Exon 4 To Exon 6 (Pair 11) | |||
| Forward (404,21) | GCAGCAGAAGGCAATGAACAG | Amplicon Size | cDNA: 298 bpgenomic: 6,607 |
| Reverse (701,22) | GTTCCTTCATGTAACGCTCCTT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -0.584 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.458 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTTGG TCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCCTT ACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAGAA GTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTGAG GAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATGAAGGAAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 287 | 403 - 689 | |
| Exon 4 To Exon 6 (Pair 12) | |||
| Forward (404,21) | GCAGCAGAAGGCAATGAACAG | Amplicon Size | cDNA: 291 bpgenomic: 6,600 |
| Reverse (694,22) | CATGTAACGCTCCTTATCTCTG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -0.584 kcal/mol | ||
| Reverse Loops | 2.32 kcal/mol | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | GCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAGGTTGG TCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCCCCCTT ACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGACCAGAA GTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTCCTGAG GAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACATG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 295 | 403 - 697 | |
| Exon 4 To Exon 6 (Pair 13) | |||
| Forward (399,22) | CAAATGCAGCAGAAGGCAATGA | Amplicon Size | cDNA: 294 bpgenomic: 6,603 |
| Reverse (692,22) | TGTAACGCTCCTTATCTCTGTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.416 kcal/mol | |
| Forward Dimers | -2.174 kcal/mol | ||
| Reverse Loops | 2.042 kcal/mol in 3' area | ||
| Reverse Dimers | -0.458 kcal/mol | ||
| Forward-Reverse Dimers | -2.078 kcal/mol | ||
| Amplicon Folding | -2.498 kcal/mol outside primersite | ||
| Amplicon Sequence | CAAATGCAGCAGAAGGCAATGAACAGAGGCATGAAGATGAGCAACGAAGTAAACGAGGAG GTTGGTCCAAAGGAAGAAAGAGGAAGAAACCTCTTCGAGACAGCAATGCACCCAAATCCC CCCTTACAGGATATGTTCGGTTCATGAATGAGCGTCGAGAACAACTTCGAGCAAAGAGAC CAGAAGTCCCATTTCCAGAAATCACAAGGATGTTAGGCAATGAATGGAGTAAACTGCCTC CTGAGGAAAAACAGCGCTACCTTGATGAAGCAGACAGAGATAAGGAGCGTTACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_018200 (HMG20A) | 289 | 403 - 691 | |
See below for some more details.
Program Output:
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