qPCR PrimerDesign Pipeline

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Design Results for IL32

(Homo Sapiens, NCBI 37 / UCSC hg19)

Settings Overview
Minimal Amplicon Size 70 Maximal Amplicon Size 300
Natrium Concentration 0.3 M Magnesium Concentration 0.015 M
Annealing Temperature 60 °C Minimal dG for primer folding 0
Minimal dG for primer dimer -3 Minimal dG for amplicon folding -3
Nr of bases considered as 3' 5 Minimal Intron Size 3000

Settings Overview
Minimal Amplicon Size	70	Maximal Amplicon Size	300
Natrium Concentration	0.3 M	Magnesium Concentration	0.015 M
Annealing Temperature	60 °C	Minimal dG for primer folding	0
Minimal dG for primer dimer	-3	Minimal dG for amplicon folding	-3
Nr of bases considered as 3'	5	Minimal Intron Size	3000

The design was not successful. No intron spanning primers were found, see below for details. You can also try to design exon-exon boundary spanning primers here.

Program Output:

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