Settings Overview | |||
---|---|---|---|
Minimal Amplicon Size | 70 | Maximal Amplicon Size | 300 |
Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
Annealing Temperature | 60 °C | Minimal dG for primer folding | 0 |
Minimal dG for primer dimer | -3 | Minimal dG for amplicon folding | -3 |
Nr of bases considered as 3' | 5 | Minimal Intron Size | 3000 |
The design was not successful. No intron spanning primers were found, see below for details. You can also try to design exon-exon boundary spanning primers here.
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