qPCR PrimerDesign Pipeline
Created By Geert Vandeweyer
Design Results for NR3C1
(Homo Sapiens, NCBI 37 / UCSC hg19)
| Settings Overview | |||
|---|---|---|---|
| Minimal Amplicon Size | 80 | Maximal Amplicon Size | 160 |
| Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
| Annealing Temperature | 60 °C | Minimal dG for primer folding | 0 |
| Minimal dG for primer dimer | -3 | Minimal dG for amplicon folding | -3 |
| Nr of bases considered as 3' | 5 | Minimal Intron Size | 3000 |
Sorting Options
Amplicon Details
Remark: Only top 100 primer pairs are shown)
| Exon 4 To Exon 5 (Pair 8) | |||
|---|---|---|---|
| Forward (2692,22) | GATGACAACTTGACTTCTCTGG | Amplicon Size | cDNA: 119 bpgenomic: 85,606 |
| Reverse (2810,20) | GTTGAGGAGCTGGATGGAGG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.847 kcal/mol | ||
| Reverse Dimers | -2.732 kcal/mol | ||
| Forward-Reverse Dimers | -1.624 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | GATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAAT GGCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCTCCTCCATCCAGCTCCTCAAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001018077 (NR3C1) | 119 | 2108 - 2226 | |
| NM_001018074 (NR3C1) | 119 | 1436 - 1554 | |
| NM_001204258 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204261 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001018075 (NR3C1) | 119 | 1339 - 1457 | |
| NM_001018076 (NR3C1) | 119 | 1232 - 1350 | |
| NM_001204264 (NR3C1) | 119 | 1606 - 1724 | |
| NM_000176 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204259 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001024094 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204263 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204262 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204260 (NR3C1) | 119 | 1606 - 1724 | |
| NM_001204265 (NR3C1) | 119 | 1606 - 1724 | |
| Exon 4 To Exon 5 (Pair 7) | |||
| Forward (2693,22) | ATGACAACTTGACTTCTCTGGG | Amplicon Size | cDNA: 96 bpgenomic: 85,583 |
| Reverse (2788,22) | AGCTTACATCTGGTCTCATGCT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.073 kcal/mol | ||
| Reverse Dimers | -1.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.133 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAATG GCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001018074 (NR3C1) | 96 | 1437 - 1532 | |
| NM_001018077 (NR3C1) | 96 | 2109 - 2204 | |
| NM_001204258 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001204261 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001018075 (NR3C1) | 96 | 1340 - 1435 | |
| NM_001204264 (NR3C1) | 96 | 1607 - 1702 | |
| NM_000176 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001018076 (NR3C1) | 96 | 1233 - 1328 | |
| NM_001204259 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001204262 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001204263 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001024094 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001204265 (NR3C1) | 96 | 1607 - 1702 | |
| NM_001204260 (NR3C1) | 96 | 1607 - 1702 | |
| Exon 4 To Exon 5 (Pair 6) | |||
| Forward (2693,22) | ATGACAACTTGACTTCTCTGGG | Amplicon Size | cDNA: 121 bpgenomic: 85,608 |
| Reverse (2813,20) | GCTGTTGAGGAGCTGGATGG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.73 kcal/mol | ||
| Reverse Dimers | -2.732 kcal/mol | ||
| Forward-Reverse Dimers | -1.895 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAATG GCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCTCCTCCATCCAGCTCCTCAACAG C | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204260 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204265 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001024094 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204262 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204263 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204259 (NR3C1) | 121 | 1607 - 1727 | |
| NM_000176 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204264 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001018076 (NR3C1) | 121 | 1233 - 1353 | |
| NM_001018075 (NR3C1) | 121 | 1340 - 1460 | |
| NM_001204261 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001204258 (NR3C1) | 121 | 1607 - 1727 | |
| NM_001018077 (NR3C1) | 121 | 2109 - 2229 | |
| NM_001018074 (NR3C1) | 121 | 1437 - 1557 | |
| NM_001020825 (NR3C1) | 121 | 1607 - 1727 | |
| Exon 4 To Exon 5 (Pair 5) | |||
| Forward (2693,22) | ATGACAACTTGACTTCTCTGGG | Amplicon Size | cDNA: 118 bpgenomic: 85,605 |
| Reverse (2810,20) | GTTGAGGAGCTGGATGGAGG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.847 kcal/mol | ||
| Reverse Dimers | -2.732 kcal/mol | ||
| Forward-Reverse Dimers | -1.624 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAATG GCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCTCCTCCATCCAGCTCCTCAAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018077 (NR3C1) | 118 | 2109 - 2226 | |
| NM_001018074 (NR3C1) | 118 | 1437 - 1554 | |
| NM_001020825 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001018075 (NR3C1) | 118 | 1340 - 1457 | |
| NM_001204258 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204261 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204259 (NR3C1) | 118 | 1607 - 1724 | |
| NM_000176 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204264 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001018076 (NR3C1) | 118 | 1233 - 1350 | |
| NM_001204260 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204265 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001024094 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204262 (NR3C1) | 118 | 1607 - 1724 | |
| NM_001204263 (NR3C1) | 118 | 1607 - 1724 | |
| Exon 4 To Exon 5 (Pair 4) | |||
| Forward (2692,22) | GATGACAACTTGACTTCTCTGG | Amplicon Size | cDNA: 99 bpgenomic: 85,586 |
| Reverse (2790,22) | AGAGCTTACATCTGGTCTCATG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.073 kcal/mol | ||
| Reverse Dimers | -2.948 kcal/mol | ||
| Forward-Reverse Dimers | -1.696 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | GATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAAT GGCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001024094 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204263 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204262 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204260 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204265 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001018076 (NR3C1) | 99 | 1232 - 1330 | |
| NM_000176 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204264 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204259 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204261 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001204258 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001018075 (NR3C1) | 99 | 1339 - 1437 | |
| NM_001020825 (NR3C1) | 99 | 1606 - 1704 | |
| NM_001018077 (NR3C1) | 99 | 2108 - 2206 | |
| NM_001018074 (NR3C1) | 99 | 1436 - 1534 | |
| Exon 4 To Exon 5 (Pair 3) | |||
| Forward (2692,22) | GATGACAACTTGACTTCTCTGG | Amplicon Size | cDNA: 97 bpgenomic: 85,584 |
| Reverse (2788,22) | AGCTTACATCTGGTCTCATGCT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.073 kcal/mol | ||
| Reverse Dimers | -1.822 kcal/mol | ||
| Forward-Reverse Dimers | -1.696 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | GATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAAT GGCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204260 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204265 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001024094 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204263 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204262 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204259 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001018076 (NR3C1) | 97 | 1232 - 1328 | |
| NM_000176 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204264 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001018075 (NR3C1) | 97 | 1339 - 1435 | |
| NM_001204261 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001204258 (NR3C1) | 97 | 1606 - 1702 | |
| NM_001018077 (NR3C1) | 97 | 2108 - 2204 | |
| NM_001018074 (NR3C1) | 97 | 1436 - 1532 | |
| NM_001020825 (NR3C1) | 97 | 1606 - 1702 | |
| Exon 4 To Exon 5 (Pair 2) | |||
| Forward (2693,22) | ATGACAACTTGACTTCTCTGGG | Amplicon Size | cDNA: 116 bpgenomic: 85,603 |
| Reverse (2808,20) | TGAGGAGCTGGATGGAGGAG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.847 kcal/mol | ||
| Reverse Dimers | -2.732 kcal/mol | ||
| Forward-Reverse Dimers | -0.962 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | ATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAATG GCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCTCCTCCATCCAGCTCCTCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018074 (NR3C1) | 116 | 1437 - 1552 | |
| NM_001018077 (NR3C1) | 116 | 2109 - 2224 | |
| NM_001020825 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001018075 (NR3C1) | 116 | 1340 - 1455 | |
| NM_001204258 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204261 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204259 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001018076 (NR3C1) | 116 | 1233 - 1348 | |
| NM_001204264 (NR3C1) | 116 | 1607 - 1722 | |
| NM_000176 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204265 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204260 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204263 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001204262 (NR3C1) | 116 | 1607 - 1722 | |
| NM_001024094 (NR3C1) | 116 | 1607 - 1722 | |
| Exon 4 To Exon 5 (Pair 1) | |||
| Forward (2692,22) | GATGACAACTTGACTTCTCTGG | Amplicon Size | cDNA: 122 bpgenomic: 85,609 |
| Reverse (2813,20) | GCTGTTGAGGAGCTGGATGG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.413 kcal/mol | |
| Forward Dimers | -0.268 kcal/mol | ||
| Reverse Loops | 1.73 kcal/mol | ||
| Reverse Dimers | -2.732 kcal/mol | ||
| Forward-Reverse Dimers | -1.895 kcal/mol | ||
| Amplicon Folding | -1.018 kcal/mol outside primersite | ||
| Amplicon Sequence | GATGACAACTTGACTTCTCTGGGGACTCTGAACTTCCCTGGTCGAACAGTTTTTTCTAAT GGCTATTCAAGCCCCAGCATGAGACCAGATGTAAGCTCTCCTCCATCCAGCTCCTCAACA GC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018076 (NR3C1) | 122 | 1232 - 1353 | |
| NM_000176 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204264 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204259 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001024094 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204263 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204262 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204260 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204265 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001020825 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001018077 (NR3C1) | 122 | 2108 - 2229 | |
| NM_001018074 (NR3C1) | 122 | 1436 - 1557 | |
| NM_001204261 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001204258 (NR3C1) | 122 | 1606 - 1727 | |
| NM_001018075 (NR3C1) | 122 | 1339 - 1460 | |
| Exon 8 To Exon 9 (Pair 18) | |||
| Forward (3427,22) | TCAAGTGCAAACCTGCTGTGTT | Amplicon Size | cDNA: 118 bpgenomic: 3,195 |
| Reverse (3544,23) | TGTGTAACTCAGAGGAAACATAC | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TCAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACT CTACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001018077 (NR3C1) | 118 | 2840 - 2957 | |
| NM_001018074 (NR3C1) | 118 | 2168 - 2285 | |
| NM_001204258 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001204261 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001018075 (NR3C1) | 118 | 2071 - 2188 | |
| NM_001018076 (NR3C1) | 118 | 1964 - 2081 | |
| NM_001204264 (NR3C1) | 118 | 2338 - 2455 | |
| NM_000176 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001204259 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001024094 (NR3C1) | 118 | 2341 - 2458 | |
| NM_001204263 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001204262 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001204260 (NR3C1) | 118 | 2338 - 2455 | |
| NM_001204265 (NR3C1) | 118 | 2338 - 2455 | |
| Exon 8 To Exon 9 (Pair 17) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 94 bpgenomic: 3,171 |
| Reverse (3543,23) | GTGTAACTCAGAGGAAACATACA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.619 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204260 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001204265 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001024094 (NR3C1) | 94 | 2364 - 2457 | |
| NM_001204263 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001204262 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001204259 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001018076 (NR3C1) | 94 | 1987 - 2080 | |
| NM_001204264 (NR3C1) | 94 | 2361 - 2454 | |
| NM_000176 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001018075 (NR3C1) | 94 | 2094 - 2187 | |
| NM_001204258 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001204261 (NR3C1) | 94 | 2361 - 2454 | |
| NM_001018077 (NR3C1) | 94 | 2863 - 2956 | |
| NM_001018074 (NR3C1) | 94 | 2191 - 2284 | |
| NM_001020825 (NR3C1) | 94 | 2361 - 2454 | |
| Exon 8 To Exon 9 (Pair 16) | |||
| Forward (3428,22) | CAAGTGCAAACCTGCTGTGTTT | Amplicon Size | cDNA: 117 bpgenomic: 3,194 |
| Reverse (3544,23) | TGTGTAACTCAGAGGAAACATAC | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | CAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTC TACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018077 (NR3C1) | 117 | 2841 - 2957 | |
| NM_001018074 (NR3C1) | 117 | 2169 - 2285 | |
| NM_001020825 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001018075 (NR3C1) | 117 | 2072 - 2188 | |
| NM_001204261 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204258 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204259 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001018076 (NR3C1) | 117 | 1965 - 2081 | |
| NM_000176 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204264 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204260 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204265 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001024094 (NR3C1) | 117 | 2342 - 2458 | |
| NM_001204263 (NR3C1) | 117 | 2339 - 2455 | |
| NM_001204262 (NR3C1) | 117 | 2339 - 2455 | |
| Exon 8 To Exon 9 (Pair 15) | |||
| Forward (3428,22) | CAAGTGCAAACCTGCTGTGTTT | Amplicon Size | cDNA: 116 bpgenomic: 3,193 |
| Reverse (3543,23) | GTGTAACTCAGAGGAAACATACA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.619 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | CAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTC TACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204261 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001204258 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001018075 (NR3C1) | 116 | 2072 - 2187 | |
| NM_001020825 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001018077 (NR3C1) | 116 | 2841 - 2956 | |
| NM_001018074 (NR3C1) | 116 | 2169 - 2284 | |
| NM_001024094 (NR3C1) | 116 | 2342 - 2457 | |
| NM_001204262 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001204263 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001204260 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001204265 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001204264 (NR3C1) | 116 | 2339 - 2454 | |
| NM_000176 (NR3C1) | 116 | 2339 - 2454 | |
| NM_001018076 (NR3C1) | 116 | 1965 - 2080 | |
| NM_001204259 (NR3C1) | 116 | 2339 - 2454 | |
| Exon 8 To Exon 9 (Pair 14) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 96 bpgenomic: 3,173 |
| Reverse (3545,23) | CTGTGTAACTCAGAGGAAACATA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -1.291 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001018077 (NR3C1) | 96 | 2863 - 2958 | |
| NM_001018074 (NR3C1) | 96 | 2191 - 2286 | |
| NM_001204258 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001204261 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001018075 (NR3C1) | 96 | 2094 - 2189 | |
| NM_000176 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001204264 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001018076 (NR3C1) | 96 | 1987 - 2082 | |
| NM_001204259 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001024094 (NR3C1) | 96 | 2364 - 2459 | |
| NM_001204262 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001204263 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001204260 (NR3C1) | 96 | 2361 - 2456 | |
| NM_001204265 (NR3C1) | 96 | 2361 - 2456 | |
| Exon 8 To Exon 9 (Pair 13) | |||
| Forward (3427,22) | TCAAGTGCAAACCTGCTGTGTT | Amplicon Size | cDNA: 117 bpgenomic: 3,194 |
| Reverse (3543,23) | GTGTAACTCAGAGGAAACATACA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.619 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TCAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACT CTACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204258 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001204261 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001018075 (NR3C1) | 117 | 2071 - 2187 | |
| NM_001020825 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001018074 (NR3C1) | 117 | 2168 - 2284 | |
| NM_001018077 (NR3C1) | 117 | 2840 - 2956 | |
| NM_001204263 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001204262 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001024094 (NR3C1) | 117 | 2341 - 2457 | |
| NM_001204265 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001204260 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001018076 (NR3C1) | 117 | 1964 - 2080 | |
| NM_000176 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001204264 (NR3C1) | 117 | 2338 - 2454 | |
| NM_001204259 (NR3C1) | 117 | 2338 - 2454 | |
| Exon 8 To Exon 9 (Pair 12) | |||
| Forward (3428,22) | CAAGTGCAAACCTGCTGTGTTT | Amplicon Size | cDNA: 118 bpgenomic: 3,195 |
| Reverse (3545,23) | CTGTGTAACTCAGAGGAAACATA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -1.291 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | CAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTC TACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018074 (NR3C1) | 118 | 2169 - 2286 | |
| NM_001018077 (NR3C1) | 118 | 2841 - 2958 | |
| NM_001020825 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001018075 (NR3C1) | 118 | 2072 - 2189 | |
| NM_001204258 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204261 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204259 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204264 (NR3C1) | 118 | 2339 - 2456 | |
| NM_000176 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001018076 (NR3C1) | 118 | 1965 - 2082 | |
| NM_001204265 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204260 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204262 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001204263 (NR3C1) | 118 | 2339 - 2456 | |
| NM_001024094 (NR3C1) | 118 | 2342 - 2459 | |
| Exon 8 To Exon 9 (Pair 11) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 95 bpgenomic: 3,172 |
| Reverse (3544,23) | TGTGTAACTCAGAGGAAACATAC | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204259 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001018076 (NR3C1) | 95 | 1987 - 2081 | |
| NM_000176 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204264 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204265 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204260 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204263 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204262 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001024094 (NR3C1) | 95 | 2364 - 2458 | |
| NM_001018074 (NR3C1) | 95 | 2191 - 2285 | |
| NM_001018077 (NR3C1) | 95 | 2863 - 2957 | |
| NM_001020825 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001018075 (NR3C1) | 95 | 2094 - 2188 | |
| NM_001204261 (NR3C1) | 95 | 2361 - 2455 | |
| NM_001204258 (NR3C1) | 95 | 2361 - 2455 | |
| Exon 8 To Exon 9 (Pair 10) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 155 bpgenomic: 3,232 |
| Reverse (3604,22) | CTGAAGAGAGAAGCAGTAAGGT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.877 kcal/mol | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.451 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGTA TCTCTGTATGAAAACCTTACTGCTTCTCTCTTCAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204261 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204258 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001018075 (NR3C1) | 155 | 2094 - 2248 | |
| NM_001020825 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001018074 (NR3C1) | 155 | 2191 - 2345 | |
| NM_001018077 (NR3C1) | 155 | 2863 - 3017 | |
| NM_001204263 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204262 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001024094 (NR3C1) | 155 | 2364 - 2518 | |
| NM_001204265 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204260 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001018076 (NR3C1) | 155 | 1987 - 2141 | |
| NM_001204264 (NR3C1) | 155 | 2361 - 2515 | |
| NM_000176 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204259 (NR3C1) | 155 | 2361 - 2515 | |
| Exon 8 To Exon 9 (Pair 9) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 96 bpgenomic: 3,173 |
| Reverse (3544,23) | TGTGTAACTCAGAGGAAACATAC | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACACA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204259 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001204264 (NR3C1) | 96 | 2360 - 2455 | |
| NM_000176 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001018076 (NR3C1) | 96 | 1986 - 2081 | |
| NM_001204260 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001204265 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001024094 (NR3C1) | 96 | 2363 - 2458 | |
| NM_001204262 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001204263 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001018077 (NR3C1) | 96 | 2862 - 2957 | |
| NM_001018074 (NR3C1) | 96 | 2190 - 2285 | |
| NM_001020825 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001018075 (NR3C1) | 96 | 2093 - 2188 | |
| NM_001204261 (NR3C1) | 96 | 2360 - 2455 | |
| NM_001204258 (NR3C1) | 96 | 2360 - 2455 | |
| Exon 8 To Exon 9 (Pair 8) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 95 bpgenomic: 3,172 |
| Reverse (3543,23) | GTGTAACTCAGAGGAAACATACA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.619 kcal/mol | ||
| Reverse Dimers | -0.72 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204261 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001204258 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001018075 (NR3C1) | 95 | 2093 - 2187 | |
| NM_001020825 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001018077 (NR3C1) | 95 | 2862 - 2956 | |
| NM_001018074 (NR3C1) | 95 | 2190 - 2284 | |
| NM_001024094 (NR3C1) | 95 | 2363 - 2457 | |
| NM_001204262 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001204263 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001204260 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001204265 (NR3C1) | 95 | 2360 - 2454 | |
| NM_000176 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001204264 (NR3C1) | 95 | 2360 - 2454 | |
| NM_001018076 (NR3C1) | 95 | 1986 - 2080 | |
| NM_001204259 (NR3C1) | 95 | 2360 - 2454 | |
| Exon 8 To Exon 9 (Pair 7) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 156 bpgenomic: 3,233 |
| Reverse (3604,22) | CTGAAGAGAGAAGCAGTAAGGT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.877 kcal/mol | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.522 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGT ATCTCTGTATGAAAACCTTACTGCTTCTCTCTTCAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001018074 (NR3C1) | 156 | 2190 - 2345 | |
| NM_001018077 (NR3C1) | 156 | 2862 - 3017 | |
| NM_001204261 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204258 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001018075 (NR3C1) | 156 | 2093 - 2248 | |
| NM_001018076 (NR3C1) | 156 | 1986 - 2141 | |
| NM_001204264 (NR3C1) | 156 | 2360 - 2515 | |
| NM_000176 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204259 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204263 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204262 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001024094 (NR3C1) | 156 | 2363 - 2518 | |
| NM_001204265 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204260 (NR3C1) | 156 | 2360 - 2515 | |
| Exon 8 To Exon 9 (Pair 6) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 157 bpgenomic: 3,234 |
| Reverse (3606,22) | ACCTGAAGAGAGAAGCAGTAAG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.666 kcal/mol | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.451 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGTA TCTCTGTATGAAAACCTTACTGCTTCTCTCTTCAGGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001020825 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001018077 (NR3C1) | 155 | 2863 - 3017 | |
| NM_001018074 (NR3C1) | 155 | 2191 - 2345 | |
| NM_001204261 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204258 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001018075 (NR3C1) | 155 | 2094 - 2248 | |
| NM_001018076 (NR3C1) | 155 | 1987 - 2141 | |
| NM_000176 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204264 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204259 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001024094 (NR3C1) | 155 | 2364 - 2518 | |
| NM_001204263 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204262 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204260 (NR3C1) | 155 | 2361 - 2515 | |
| NM_001204265 (NR3C1) | 157 | 2361 - 2517 | |
| Exon 8 To Exon 9 (Pair 5) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 158 bpgenomic: 3,235 |
| Reverse (3606,22) | ACCTGAAGAGAGAAGCAGTAAG | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.666 kcal/mol | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.522 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGT ATCTCTGTATGAAAACCTTACTGCTTCTCTCTTCAGGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204258 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204261 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001018075 (NR3C1) | 156 | 2093 - 2248 | |
| NM_001020825 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001018077 (NR3C1) | 156 | 2862 - 3017 | |
| NM_001018074 (NR3C1) | 156 | 2190 - 2345 | |
| NM_001024094 (NR3C1) | 156 | 2363 - 2518 | |
| NM_001204263 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204262 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204260 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204265 (NR3C1) | 158 | 2360 - 2517 | |
| NM_001018076 (NR3C1) | 156 | 1986 - 2141 | |
| NM_001204264 (NR3C1) | 156 | 2360 - 2515 | |
| NM_000176 (NR3C1) | 156 | 2360 - 2515 | |
| NM_001204259 (NR3C1) | 156 | 2360 - 2515 | |
| Exon 8 To Exon 9 (Pair 4) | |||
| Forward (3427,22) | TCAAGTGCAAACCTGCTGTGTT | Amplicon Size | cDNA: 119 bpgenomic: 3,196 |
| Reverse (3545,23) | CTGTGTAACTCAGAGGAAACATA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.329 kcal/mol | |
| Forward Dimers | -1.212 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -1.291 kcal/mol | ||
| Forward-Reverse Dimers | -2.426 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TCAAGTGCAAACCTGCTGTGTTTTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACT CTACCCTGCATGTACGACCAATGTAAACACATGCTGTATGTTTCCTCTGAGTTACACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001018076 (NR3C1) | 119 | 1964 - 2082 | |
| NM_001204264 (NR3C1) | 119 | 2338 - 2456 | |
| NM_000176 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001204259 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001204263 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001204262 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001024094 (NR3C1) | 119 | 2341 - 2459 | |
| NM_001204265 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001204260 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001020825 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001018074 (NR3C1) | 119 | 2168 - 2286 | |
| NM_001018077 (NR3C1) | 119 | 2840 - 2958 | |
| NM_001204261 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001204258 (NR3C1) | 119 | 2338 - 2456 | |
| NM_001018075 (NR3C1) | 119 | 2071 - 2189 | |
| Exon 8 To Exon 9 (Pair 3) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 155 bpgenomic: 3,232 |
| Reverse (3603,23) | TGAAGAGAGAAGCAGTAAGGTTT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 2.496 kcal/mol in 3' area | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.522 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGT ATCTCTGTATGAAAACCTTACTGCTTCTCTCTTCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204260 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001204265 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001024094 (NR3C1) | 155 | 2363 - 2517 | |
| NM_001204263 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001204262 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001204259 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001018076 (NR3C1) | 155 | 1986 - 2140 | |
| NM_000176 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001204264 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001018075 (NR3C1) | 155 | 2093 - 2247 | |
| NM_001204258 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001204261 (NR3C1) | 155 | 2360 - 2514 | |
| NM_001018077 (NR3C1) | 155 | 2862 - 3016 | |
| NM_001018074 (NR3C1) | 155 | 2190 - 2344 | |
| NM_001020825 (NR3C1) | 155 | 2360 - 2514 | |
| Exon 8 To Exon 9 (Pair 2) | |||
| Forward (3449,24) | TTGCTCCTGATCTGATTATTAATG | Amplicon Size | cDNA: 97 bpgenomic: 3,174 |
| Reverse (3545,23) | CTGTGTAACTCAGAGGAAACATA | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 1.348 kcal/mol | ||
| Reverse Dimers | -1.291 kcal/mol | ||
| Forward-Reverse Dimers | -2.188 kcal/mol | ||
| Amplicon Folding | -0.625 kcal/mol outside primersite | ||
| Amplicon Sequence | TTGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAAT GTAAACACATGCTGTATGTTTCCTCTGAGTTACACAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204263 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001204262 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001024094 (NR3C1) | 97 | 2363 - 2459 | |
| NM_001204265 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001204260 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001018076 (NR3C1) | 97 | 1986 - 2082 | |
| NM_001204264 (NR3C1) | 97 | 2360 - 2456 | |
| NM_000176 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001204259 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001204261 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001204258 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001018075 (NR3C1) | 97 | 2093 - 2189 | |
| NM_001020825 (NR3C1) | 97 | 2360 - 2456 | |
| NM_001018074 (NR3C1) | 97 | 2190 - 2286 | |
| NM_001018077 (NR3C1) | 97 | 2862 - 2958 | |
| Exon 8 To Exon 9 (Pair 1) | |||
| Forward (3450,24) | TGCTCCTGATCTGATTATTAATGA | Amplicon Size | cDNA: 154 bpgenomic: 3,231 |
| Reverse (3603,23) | TGAAGAGAGAAGCAGTAAGGTTT | Nr. of Isoforms | 15 out of 15 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.619 kcal/mol in 3' area | |
| Forward Dimers | -1.46 kcal/mol | ||
| Reverse Loops | 2.496 kcal/mol in 3' area | ||
| Reverse Dimers | -0.584 kcal/mol | ||
| Forward-Reverse Dimers | -2.451 kcal/mol | ||
| Amplicon Folding | -0.632 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTCCTGATCTGATTATTAATGAGCAGAGAATGACTCTACCCTGCATGTACGACCAATG TAAACACATGCTGTATGTTTCCTCTGAGTTACACAGGCTTCAGGTATCTTATGAAGAGTA TCTCTGTATGAAAACCTTACTGCTTCTCTCTTCA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_001204258 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001204261 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001018075 (NR3C1) | 154 | 2094 - 2247 | |
| NM_001020825 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001018074 (NR3C1) | 154 | 2191 - 2344 | |
| NM_001018077 (NR3C1) | 154 | 2863 - 3016 | |
| NM_001204263 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001204262 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001024094 (NR3C1) | 154 | 2364 - 2517 | |
| NM_001204265 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001204260 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001018076 (NR3C1) | 154 | 1987 - 2140 | |
| NM_001204264 (NR3C1) | 154 | 2361 - 2514 | |
| NM_000176 (NR3C1) | 154 | 2361 - 2514 | |
| NM_001204259 (NR3C1) | 154 | 2361 - 2514 | |
See below for some more details.
Program Output:
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