qPCR PrimerDesign Pipeline
Created By Geert Vandeweyer
Design Results for mmp13
(Homo Sapiens, NCBI 37 / UCSC hg19)
| Settings Overview | |||
|---|---|---|---|
| Minimal Amplicon Size | 70 | Maximal Amplicon Size | 400 |
| Natrium Concentration | 0.3 M | Magnesium Concentration | 0.015 M |
| Annealing Temperature | 60 °C | Minimal dG for primer folding | -2 |
| Minimal dG for primer dimer | -4 | Minimal dG for amplicon folding | -4 |
| Nr of bases considered as 3' | 5 | Minimal Intron Size | 0 |
Sorting Options
Amplicon Details
Remark: Only top 100 primer pairs are shown)
| Exon 7 To Exon 10 (Pair 1) | |||
|---|---|---|---|
| Forward (1019,21) | TCCCAACCGTATTGATGCTGC | Amplicon Size | cDNA: 373 bpgenomic: 4,767 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.713 kcal/mol | |
| Forward Dimers | -0.692 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.834 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | TCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTTCAG AGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAAAAT ATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGA TACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCA TATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAA AGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTT TGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 373 | 1019 - 1391 | |
| Exon 7 To Exon 10 (Pair 2) | |||
| Forward (1036,21) | CTGCATATGAGCACCCTTCTC | Amplicon Size | cDNA: 356 bpgenomic: 4,750 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.775 kcal/mol | |
| Forward Dimers | -2.37 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.688 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | CTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTTCAGAGGTAGAAAATTTTGGG CTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAAAATATCTGAACTGGGTCTTC CAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCC TGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACT ATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATG AGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 356 | 1036 - 1391 | |
| Exon 7 To Exon 10 (Pair 3) | |||
| Forward (1034,21) | TGCTGCATATGAGCACCCTTC | Amplicon Size | cDNA: 358 bpgenomic: 4,752 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.775 kcal/mol | |
| Forward Dimers | -2.37 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.688 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTTCAGAGGTAGAAAATTTTG GGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAAAATATCTGAACTGGGTCT TCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCT CCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGA CTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTA TGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 358 | 1034 - 1391 | |
| Exon 7 To Exon 10 (Pair 4) | |||
| Forward (1006,21) | TTTGGCCAGAACTTCCCAACC | Amplicon Size | cDNA: 386 bpgenomic: 4,780 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.285 kcal/mol in 3' area | |
| Forward Dimers | -3.894 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.14 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | TTTGGCCAGAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCA TCTTCATCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTT ATCCCAAAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTG TTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATG ATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAG GAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACG GACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 386 | 1006 - 1391 | |
| Exon 7 To Exon 10 (Pair 5) | |||
| Forward (1009,20) | GGCCAGAACTTCCCAACCGT | Amplicon Size | cDNA: 383 bpgenomic: 4,777 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.131 kcal/mol | |
| Forward Dimers | -2.61 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.14 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | GGCCAGAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCT TCATCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATC CCAAAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTC ACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATG ATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAA TTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGAC CCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 383 | 1009 - 1391 | |
| Exon 7 To Exon 10 (Pair 6) | |||
| Forward (1010,21) | GCCAGAACTTCCCAACCGTAT | Amplicon Size | cDNA: 382 bpgenomic: 4,776 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.131 kcal/mol | |
| Forward Dimers | -0.646 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.14 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | GCCAGAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTT CATCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCC CAAAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCA CTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGA TACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAAT TGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACC CATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 382 | 1010 - 1391 | |
| Exon 7 To Exon 10 (Pair 7) | |||
| Forward (1037,22) | TGCATATGAGCACCCTTCTCAT | Amplicon Size | cDNA: 355 bpgenomic: 4,749 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.775 kcal/mol | |
| Forward Dimers | -2.37 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.688 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | TGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTTCAGAGGTAGAAAATTTTGGGC TCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAAAATATCTGAACTGGGTCTTCC AAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCT GTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTA TCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGA GAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 355 | 1037 - 1391 | |
| Exon 7 To Exon 10 (Pair 8) | |||
| Forward (1008,20) | TGGCCAGAACTTCCCAACCG | Amplicon Size | cDNA: 384 bpgenomic: 4,778 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.757 kcal/mol in 3' area | |
| Forward Dimers | -3.752 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.14 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | TGGCCAGAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATC TTCATCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTAT CCCAAAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTT CACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGAT GATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGA ATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGA CCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 384 | 1008 - 1391 | |
| Exon 7 To Exon 10 (Pair 9) | |||
| Forward (1035,21) | GCTGCATATGAGCACCCTTCT | Amplicon Size | cDNA: 357 bpgenomic: 4,751 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.775 kcal/mol | |
| Forward Dimers | -2.37 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.688 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | GCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTTCAGAGGTAGAAAATTTTGG GCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAAAATATCTGAACTGGGTCTT CCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTC CTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGAC TATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTAT GAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 357 | 1035 - 1391 | |
| Exon 7 To Exon 10 (Pair 10) | |||
| Forward (1012,22) | CAGAACTTCCCAACCGTATTGA | Amplicon Size | cDNA: 380 bpgenomic: 4,774 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.713 kcal/mol in 3' area | |
| Forward Dimers | -0.646 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.997 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | CAGAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCA TCTTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCA AAAAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACT TTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATA CTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTG GTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCA TACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 380 | 1012 - 1391 | |
| Exon 7 To Exon 10 (Pair 11) | |||
| Forward (1015,22) | AACTTCCCAACCGTATTGATGC | Amplicon Size | cDNA: 377 bpgenomic: 4,771 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.713 kcal/mol in 3' area | |
| Forward Dimers | -0.237 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.834 kcal/mol in 3' area | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | AACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCT TCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAA AAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTG AGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTA ACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTG ATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATAC AGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 377 | 1015 - 1391 | |
| Exon 7 To Exon 10 (Pair 12) | |||
| Forward (1016,22) | ACTTCCCAACCGTATTGATGCT | Amplicon Size | cDNA: 376 bpgenomic: 4,770 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.713 kcal/mol | |
| Forward Dimers | -0.237 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.834 kcal/mol | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | ACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATCTT CAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAAAA AATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGA GGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAA CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 376 | 1016 - 1391 | |
| Exon 7 To Exon 10 (Pair 13) | |||
| Forward (1014,22) | GAACTTCCCAACCGTATTGATG | Amplicon Size | cDNA: 378 bpgenomic: 4,772 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.713 kcal/mol in 3' area | |
| Forward Dimers | -0.237 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.834 kcal/mol in 3' area | ||
| Amplicon Folding | -2.555 kcal/mol outside primersite | ||
| Amplicon Sequence | GAACTTCCCAACCGTATTGATGCTGCATATGAGCACCCTTCTCATGACCTCATCTTCATC TTCAGAGGTAGAAAATTTTGGGCTCTTAATGGTTATGACATTCTGGAAGGTTATCCCAAA AAAATATCTGAACTGGGTCTTCCAAAAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTT GAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACT AACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGT GATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATA CAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 378 | 1014 - 1391 | |
| Exon 8 To Exon 10 (Pair 1) | |||
| Forward (1204,21) | GCAAGACTCTCCTGTTCTCAG | Amplicon Size | cDNA: 188 bpgenomic: 3,608 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.397 kcal/mol in 3' area | |
| Forward Dimers | -0.717 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.064 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | GCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTA TGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAG ATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAAT ACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 188 | 1204 - 1391 | |
| Exon 8 To Exon 10 (Pair 2) | |||
| Forward (1207,22) | AGACTCTCCTGTTCTCAGGAAA | Amplicon Size | cDNA: 185 bpgenomic: 3,605 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.848 kcal/mol in 3' area | |
| Forward Dimers | -1.877 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.816 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | AGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGG ATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATG CTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACA GCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 185 | 1207 - 1391 | |
| Exon 8 To Exon 10 (Pair 3) | |||
| Forward (1170,23) | AAGATAAGTGCAGCTGTTCACTT | Amplicon Size | cDNA: 321 bpgenomic: 3,741 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAAC CAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATA GAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTAT ATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTT CGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTT AAATCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 321 | 1170 - 1490 | |
| Exon 8 To Exon 10 (Pair 4) | |||
| Forward (1173,23) | ATAAGTGCAGCTGTTCACTTTGA | Amplicon Size | cDNA: 317 bpgenomic: 3,737 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAG GTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAA GAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATC TATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGC GTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAA TCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 317 | 1173 - 1489 | |
| Exon 8 To Exon 10 (Pair 5) | |||
| Forward (1159,24) | AAGAAGTTAAGAAGATAAGTGCAG | Amplicon Size | cDNA: 331 bpgenomic: 3,751 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.131 kcal/mol | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol in 3' area | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGT TCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATC CGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGA AAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTA ACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAA AATTGTTATTTAAATCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 331 | 1159 - 1489 | |
| Exon 8 To Exon 10 (Pair 6) | |||
| Forward (1210,21) | CTCTCCTGTTCTCAGGAAACC | Amplicon Size | cDNA: 182 bpgenomic: 3,602 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.848 kcal/mol | |
| Forward Dimers | -1.877 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -0.816 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | CTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATA AAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTG TCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCA TC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 182 | 1210 - 1391 | |
| Exon 8 To Exon 10 (Pair 7) | |||
| Forward (1173,23) | ATAAGTGCAGCTGTTCACTTTGA | Amplicon Size | cDNA: 219 bpgenomic: 3,639 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.236 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAG GTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAA GAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATC TATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 219 | 1173 - 1391 | |
| Exon 8 To Exon 10 (Pair 8) | |||
| Forward (1175,22) | AAGTGCAGCTGTTCACTTTGAG | Amplicon Size | cDNA: 315 bpgenomic: 3,735 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.813 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGT CTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGA AGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTA TTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGT CATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATC CTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 315 | 1175 - 1489 | |
| Exon 8 To Exon 10 (Pair 9) | |||
| Forward (1205,21) | CAAGACTCTCCTGTTCTCAGG | Amplicon Size | cDNA: 187 bpgenomic: 3,607 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.037 kcal/mol in 3' area | |
| Forward Dimers | -1.066 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.064 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | CAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTAT GGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGA TGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATA CAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 187 | 1205 - 1391 | |
| Exon 8 To Exon 10 (Pair 10) | |||
| Forward (1161,23) | GAAGTTAAGAAGATAAGTGCAGC | Amplicon Size | cDNA: 231 bpgenomic: 3,651 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.585 kcal/mol in 3' area | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.128 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | GAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTC TCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCG AGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAA AATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 231 | 1161 - 1391 | |
| Exon 8 To Exon 10 (Pair 11) | |||
| Forward (1171,23) | AGATAAGTGCAGCTGTTCACTTT | Amplicon Size | cDNA: 221 bpgenomic: 3,641 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.236 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | AGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACC AGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAG AAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATA TCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 221 | 1171 - 1391 | |
| Exon 8 To Exon 10 (Pair 12) | |||
| Forward (1212,21) | CTCCTGTTCTCAGGAAACCAG | Amplicon Size | cDNA: 180 bpgenomic: 3,600 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.848 kcal/mol | |
| Forward Dimers | -1.877 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.086 kcal/mol in 3' area | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | CTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAA GACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTC TATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 180 | 1212 - 1391 | |
| Exon 8 To Exon 10 (Pair 13) | |||
| Forward (1159,24) | AAGAAGTTAAGAAGATAAGTGCAG | Amplicon Size | cDNA: 332 bpgenomic: 3,752 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.131 kcal/mol | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol in 3' area | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGT TCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATC CGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGA AAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTA ACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAA AATTGTTATTTAAATCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 332 | 1159 - 1490 | |
| Exon 8 To Exon 10 (Pair 14) | |||
| Forward (1213,21) | TCCTGTTCTCAGGAAACCAGG | Amplicon Size | cDNA: 179 bpgenomic: 3,599 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.848 kcal/mol | |
| Forward Dimers | -1.877 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.086 kcal/mol in 3' area | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | TCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAG ACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCT ATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 179 | 1213 - 1391 | |
| Exon 8 To Exon 10 (Pair 15) | |||
| Forward (1161,23) | GAAGTTAAGAAGATAAGTGCAGC | Amplicon Size | cDNA: 329 bpgenomic: 3,749 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.585 kcal/mol in 3' area | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol in 3' area | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | GAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTC TCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCG AGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAA AATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAAC CGTATTGTTCGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAA TTGTTATTTAAATCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 329 | 1161 - 1489 | |
| Exon 8 To Exon 10 (Pair 16) | |||
| Forward (1159,24) | AAGAAGTTAAGAAGATAAGTGCAG | Amplicon Size | cDNA: 233 bpgenomic: 3,653 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.131 kcal/mol | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.128 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGT TCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATC CGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGA AAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 233 | 1159 - 1391 | |
| Exon 8 To Exon 10 (Pair 17) | |||
| Forward (1175,22) | AAGTGCAGCTGTTCACTTTGAG | Amplicon Size | cDNA: 316 bpgenomic: 3,736 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.813 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGT CTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGA AGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTA TTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGT CATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATC CTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 316 | 1175 - 1490 | |
| Exon 8 To Exon 10 (Pair 18) | |||
| Forward (1161,23) | GAAGTTAAGAAGATAAGTGCAGC | Amplicon Size | cDNA: 330 bpgenomic: 3,750 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 2.585 kcal/mol in 3' area | |
| Forward Dimers | -1.072 kcal/mol in 3' area | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol in 3' area | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | GAAGTTAAGAAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTC TCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCG AGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAA AATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAAC CGTATTGTTCGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAA TTGTTATTTAAATCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 330 | 1161 - 1490 | |
| Exon 8 To Exon 10 (Pair 19) | |||
| Forward (1177,21) | GTGCAGCTGTTCACTTTGAGG | Amplicon Size | cDNA: 215 bpgenomic: 3,635 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.651 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.236 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCT GGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAG ACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATT TTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 215 | 1177 - 1391 | |
| Exon 8 To Exon 10 (Pair 20) | |||
| Forward (1203,21) | GGCAAGACTCTCCTGTTCTCA | Amplicon Size | cDNA: 189 bpgenomic: 3,609 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.665 kcal/mol | |
| Forward Dimers | -0.717 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.351 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | GGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATT ATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTA GATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAA TACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 189 | 1203 - 1391 | |
| Exon 8 To Exon 10 (Pair 21) | |||
| Forward (1177,21) | GTGCAGCTGTTCACTTTGAGG | Amplicon Size | cDNA: 313 bpgenomic: 3,733 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.651 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.813 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCT GGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAG ACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATT TTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCA TGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCT GAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 313 | 1177 - 1489 | |
| Exon 8 To Exon 10 (Pair 22) | |||
| Forward (1170,23) | AAGATAAGTGCAGCTGTTCACTT | Amplicon Size | cDNA: 222 bpgenomic: 3,642 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.236 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAAC CAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATA GAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTAT ATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 222 | 1170 - 1391 | |
| Exon 8 To Exon 10 (Pair 23) | |||
| Forward (1171,23) | AGATAAGTGCAGCTGTTCACTTT | Amplicon Size | cDNA: 319 bpgenomic: 3,739 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACC AGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAG AAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATA TCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTC GCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTA AATCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 319 | 1171 - 1489 | |
| Exon 8 To Exon 10 (Pair 24) | |||
| Forward (1170,23) | AAGATAAGTGCAGCTGTTCACTT | Amplicon Size | cDNA: 320 bpgenomic: 3,740 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAAC CAGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATA GAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTAT ATCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTT CGCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTT AAATCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 320 | 1170 - 1489 | |
| Exon 8 To Exon 10 (Pair 25) | |||
| Forward (1177,21) | GTGCAGCTGTTCACTTTGAGG | Amplicon Size | cDNA: 314 bpgenomic: 3,734 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 1.651 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.813 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | GTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGTCT GGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAG ACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATT TTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCA TGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCT GAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 314 | 1177 - 1490 | |
| Exon 8 To Exon 10 (Pair 26) | |||
| Forward (1171,23) | AGATAAGTGCAGCTGTTCACTTT | Amplicon Size | cDNA: 320 bpgenomic: 3,740 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | AGATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACC AGGTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAG AAGAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATA TCTATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTC GCGTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTA AATCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 320 | 1171 - 1490 | |
| Exon 8 To Exon 10 (Pair 27) | |||
| Forward (1175,22) | AAGTGCAGCTGTTCACTTTGAG | Amplicon Size | cDNA: 217 bpgenomic: 3,637 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.438 kcal/mol | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.236 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol outside primersite | ||
| Amplicon Sequence | AAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAGGT CTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAAGA AGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTA TTTTTTCAACGGACCCATACAGTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 217 | 1175 - 1391 | |
| Exon 8 To Exon 10 (Pair 28) | |||
| Forward (1206,22) | AAGACTCTCCTGTTCTCAGGAA | Amplicon Size | cDNA: 186 bpgenomic: 3,606 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | -0.848 kcal/mol in 3' area | |
| Forward Dimers | -1.877 kcal/mol in 3' area | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.064 kcal/mol | ||
| Amplicon Folding | -1.795 kcal/mol inside primersite | ||
| Amplicon Sequence | AAGACTCTCCTGTTCTCAGGAAACCAGGTCTGGAGATATGATGATACTAACCATATTATG GATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGATAAAGTAGAT GCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACAGTTTGAATAC AGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 186 | 1206 - 1391 | |
| Exon 8 To Exon 10 (Pair 29) | |||
| Forward (1173,23) | ATAAGTGCAGCTGTTCACTTTGA | Amplicon Size | cDNA: 318 bpgenomic: 3,738 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.304 kcal/mol in 3' area | |
| Forward Dimers | -3.624 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.911 kcal/mol | ||
| Amplicon Folding | -2.454 kcal/mol outside primersite | ||
| Amplicon Sequence | ATAAGTGCAGCTGTTCACTTTGAGGATACAGGCAAGACTCTCCTGTTCTCAGGAAACCAG GTCTGGAGATATGATGATACTAACCATATTATGGATAAAGACTATCCGAGACTAATAGAA GAAGACTTCCCAGGAATTGGTGATAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATC TATTTTTTCAACGGACCCATACAGTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGC GTCATGCCAGCAAATTCCATTTTGTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAA TCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 318 | 1173 - 1490 | |
| Exon 9 To Exon 10 (Pair 1) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 140 bpgenomic: 1,419 |
| Reverse (1395,23) | TCCAGATGCTGTATTCAAACTGT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.681 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.659 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 140 | 1256 - 1395 | |
| Exon 9 To Exon 10 (Pair 2) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 282 bpgenomic: 1,561 |
| Reverse (1537,21) | CTCCTGATAGCTCTTCTTCCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.44 kcal/mol in 3' area | ||
| Reverse Dimers | -2.288 kcal/mol | ||
| Forward-Reverse Dimers | -1.383 kcal/mol in 3' area | ||
| Amplicon Folding | -0.885 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGGGTAAT ACTTCCAGAAGTGCGGGGTAGGGGAAGAAGAGCTATCAGGAG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 282 | 1256 - 1537 | |
| Exon 9 To Exon 10 (Pair 3) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 143 bpgenomic: 1,422 |
| Reverse (1398,23) | TACTCCAGATGCTGTATTCAAAC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.65 kcal/mol | ||
| Forward-Reverse Dimers | -1.659 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 143 | 1256 - 1398 | |
| Exon 9 To Exon 10 (Pair 4) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 139 bpgenomic: 1,418 |
| Reverse (1394,23) | CCAGATGCTGTATTCAAACTGTA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.354 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 139 | 1256 - 1394 | |
| Exon 9 To Exon 10 (Pair 5) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 234 bpgenomic: 1,513 |
| Reverse (1489,24) | CCAAATGCTCTTCAGGATTTAAAT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.533 kcal/mol in 3' area | ||
| Amplicon Folding | -0.659 kcal/mol outside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 234 | 1256 - 1489 | |
| Exon 9 To Exon 10 (Pair 6) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 136 bpgenomic: 1,415 |
| Reverse (1391,23) | GATGCTGTATTCAAACTGTATGG | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.826 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.282 kcal/mol in 3' area | ||
| Amplicon Folding | 0.043 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 136 | 1256 - 1391 | |
| Exon 9 To Exon 10 (Pair 7) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 142 bpgenomic: 1,421 |
| Reverse (1397,23) | ACTCCAGATGCTGTATTCAAACT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol | ||
| Reverse Dimers | -0.65 kcal/mol | ||
| Forward-Reverse Dimers | -1.659 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 142 | 1256 - 1397 | |
| Exon 9 To Exon 10 (Pair 8) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 400 bpgenomic: 1,679 |
| Reverse (1655,23) | AGAGATCCTCCATTTCATTACTC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.718 kcal/mol | ||
| Reverse Dimers | -0.739 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.461 kcal/mol | ||
| Amplicon Folding | -3.574 kcal/mol outside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGGGTAAT ACTTCCAGAAGTGCGGGGTAGGGGAAGAAGAGCTATCAGGAGAAAGCTTGGTTCTGTGAA CAAGCTTCAGTAAGTTATCTTTGAATATGTAGTATCTATATGACTATGCGTGGCTGGAAC CACATTGAAGAATGTTAGAGTAATGAAATGGAGGATCTCT | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 400 | 1256 - 1655 | |
| Exon 9 To Exon 10 (Pair 9) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 287 bpgenomic: 1,566 |
| Reverse (1542,22) | GCTTTCTCCTGATAGCTCTTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.643 kcal/mol | ||
| Reverse Dimers | -2.288 kcal/mol | ||
| Forward-Reverse Dimers | -1.585 kcal/mol | ||
| Amplicon Folding | -0.885 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGGGTAAT ACTTCCAGAAGTGCGGGGTAGGGGAAGAAGAGCTATCAGGAGAAAGC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 287 | 1256 - 1542 | |
| Exon 9 To Exon 10 (Pair 10) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 284 bpgenomic: 1,563 |
| Reverse (1539,22) | TTCTCCTGATAGCTCTTCTTCC | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.44 kcal/mol in 3' area | ||
| Reverse Dimers | -2.288 kcal/mol | ||
| Forward-Reverse Dimers | -1.383 kcal/mol in 3' area | ||
| Amplicon Folding | -0.885 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGGGTAAT ACTTCCAGAAGTGCGGGGTAGGGGAAGAAGAGCTATCAGGAGAA | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 284 | 1256 - 1539 | |
| Exon 9 To Exon 10 (Pair 11) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 399 bpgenomic: 1,678 |
| Reverse (1654,23) | GAGATCCTCCATTTCATTACTCT | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.735 kcal/mol | ||
| Reverse Dimers | -0.739 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -2.461 kcal/mol | ||
| Amplicon Folding | -3.574 kcal/mol outside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGGGTAAT ACTTCCAGAAGTGCGGGGTAGGGGAAGAAGAGCTATCAGGAGAAAGCTTGGTTCTGTGAA CAAGCTTCAGTAAGTTATCTTTGAATATGTAGTATCTATATGACTATGCGTGGCTGGAAC CACATTGAAGAATGTTAGAGTAATGAAATGGAGGATCTC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 399 | 1256 - 1654 | |
| Exon 9 To Exon 10 (Pair 12) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 235 bpgenomic: 1,514 |
| Reverse (1490,23) | CCCAAATGCTCTTCAGGATTTAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 0.275 kcal/mol in 3' area | ||
| Reverse Dimers | -1.849 kcal/mol in 3' area | ||
| Forward-Reverse Dimers | -1.635 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol outside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAACCGTATTGTTCGCGTCATGCCAGCAAATTCCATTTT GTGGTGTTAAGTGTCTTTTTAAAAATTGTTATTTAAATCCTGAAGAGCATTTGGG | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 235 | 1256 - 1490 | |
| Exon 9 To Exon 10 (Pair 13) | |||
| Forward (1256,24) | CCATATTATGGATAAAGACTATCC | Amplicon Size | cDNA: 145 bpgenomic: 1,424 |
| Reverse (1400,23) | GTTACTCCAGATGCTGTATTCAA | Nr. of Isoforms | 1 out of 1 (by amplicon BlastN) |
| Folding Energies | Forward Loops | 0.043 kcal/mol | |
| Forward Dimers | -2.387 kcal/mol | ||
| Reverse Loops | 1.633 kcal/mol in 3' area | ||
| Reverse Dimers | -0.65 kcal/mol | ||
| Forward-Reverse Dimers | -1.659 kcal/mol | ||
| Amplicon Folding | -0.659 kcal/mol inside primersite | ||
| Amplicon Sequence | CCATATTATGGATAAAGACTATCCGAGACTAATAGAAGAAGACTTCCCAGGAATTGGTGA TAAAGTAGATGCTGTCTATGAGAAAAATGGTTATATCTATTTTTTCAACGGACCCATACA GTTTGAATACAGCATCTGGAGTAAC | All Amplified targets (by primer blast) | Transcript | Size | Position on transcript |
| NM_002427 (MMP13) | 145 | 1256 - 1400 | |
See below for some more details.
Program Output:
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