Design Results for nell1

(Homo Sapiens, NCBI 37 / UCSC hg19)

Settings Overview
Minimal Amplicon Size70Maximal Amplicon Size300
Natrium Concentration0.3 MMagnesium Concentration0.015 M
Annealing Temperature60 °CMinimal dG for primer folding0
Minimal dG for primer dimer-1.5 Minimal dG for amplicon folding-1.5
Nr of bases considered as 3'5Minimal Intron Size3000

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Amplicon Details

Remark: Only top 100 primer pairs are shown)

Exon 3 To Exon 4 (Pair 1)
Forward (368,20)GGAGTTGCTCAGGTGTCTGGAmplicon SizecDNA: 78 bpgenomic: 105,697
Reverse (445,23)TGCATGGATCTCTCTTTCTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.07 kcal/mol in 3' area
Forward Dimers-0.293 kcal/mol
Reverse Loops2.336 kcal/mol in 3' area
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACATA
GAAAGAGAGATCCATGCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)78283 - 360
NM_006157 (NELL1)78283 - 360
Exon 3 To Exon 4 (Pair 2)
Forward (365,21)CTTGGAGTTGCTCAGGTGTCTAmplicon SizecDNA: 81 bpgenomic: 105,700
Reverse (445,23)TGCATGGATCTCTCTTTCTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.458 kcal/mol
Forward Dimers-0.293 kcal/mol
Reverse Loops2.336 kcal/mol in 3' area
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceCTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGAC
ATAGAAAGAGAGATCCATGCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)81280 - 360
NM_006157 (NELL1)81280 - 360
Exon 3 To Exon 4 (Pair 3)
Forward (306,20)GGATGGACCCTGACCTTCAGAmplicon SizecDNA: 142 bpgenomic: 105,761
Reverse (447,22)GCTGCATGGATCTCTCTTTCTANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.057 kcal/mol in 3' area
Forward Dimers-0.566 kcal/mol
Reverse Loops2.093 kcal/mol
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-1.232 kcal/mol outside primersite
Amplicon SequenceGGATGGACCCTGACCTTCAGATGGATATCGTCACCGAGCTTGACCTTGTGAACACCACCC
TTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACA
TAGAAAGAGAGATCCATGCAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)142221 - 362
NM_006157 (NELL1)142221 - 362
Exon 3 To Exon 4 (Pair 4)
Forward (360,20)CCACCCTTGGAGTTGCTCAGAmplicon SizecDNA: 86 bpgenomic: 105,705
Reverse (445,23)TGCATGGATCTCTCTTTCTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.492 kcal/mol
Forward Dimers-0.691 kcal/mol
Reverse Loops2.336 kcal/mol in 3' area
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.38 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceCCACCCTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTC
AAGACATAGAAAGAGAGATCCATGCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)86275 - 360
NM_006157 (NELL1)86275 - 360
Exon 3 To Exon 4 (Pair 5)
Forward (365,21)CTTGGAGTTGCTCAGGTGTCTAmplicon SizecDNA: 83 bpgenomic: 105,702
Reverse (447,22)GCTGCATGGATCTCTCTTTCTANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.458 kcal/mol
Forward Dimers-0.293 kcal/mol
Reverse Loops2.093 kcal/mol
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceCTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGAC
ATAGAAAGAGAGATCCATGCAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)83280 - 362
NM_006157 (NELL1)83280 - 362
Exon 3 To Exon 4 (Pair 6)
Forward (306,20)GGATGGACCCTGACCTTCAGAmplicon SizecDNA: 140 bpgenomic: 105,759
Reverse (445,23)TGCATGGATCTCTCTTTCTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.057 kcal/mol in 3' area
Forward Dimers-0.566 kcal/mol
Reverse Loops2.336 kcal/mol in 3' area
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-1.232 kcal/mol outside primersite
Amplicon SequenceGGATGGACCCTGACCTTCAGATGGATATCGTCACCGAGCTTGACCTTGTGAACACCACCC
TTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACA
TAGAAAGAGAGATCCATGCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)140221 - 360
NM_006157 (NELL1)140221 - 360
Exon 3 To Exon 4 (Pair 7)
Forward (368,20)GGAGTTGCTCAGGTGTCTGGAmplicon SizecDNA: 80 bpgenomic: 105,699
Reverse (447,22)GCTGCATGGATCTCTCTTTCTANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.07 kcal/mol in 3' area
Forward Dimers-0.293 kcal/mol
Reverse Loops2.093 kcal/mol
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACATA
GAAAGAGAGATCCATGCAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)80283 - 362
NM_006157 (NELL1)80283 - 362
Exon 3 To Exon 4 (Pair 8)
Forward (366,21)TTGGAGTTGCTCAGGTGTCTGAmplicon SizecDNA: 80 bpgenomic: 105,699
Reverse (445,23)TGCATGGATCTCTCTTTCTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.232 kcal/mol in 3' area
Forward Dimers-0.293 kcal/mol
Reverse Loops2.336 kcal/mol in 3' area
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACA
TAGAAAGAGAGATCCATGCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)80281 - 360
NM_006157 (NELL1)80281 - 360
Exon 3 To Exon 4 (Pair 9)
Forward (360,20)CCACCCTTGGAGTTGCTCAGAmplicon SizecDNA: 88 bpgenomic: 105,707
Reverse (447,22)GCTGCATGGATCTCTCTTTCTANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.492 kcal/mol
Forward Dimers-0.691 kcal/mol
Reverse Loops2.093 kcal/mol
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.463 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceCCACCCTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTC
AAGACATAGAAAGAGAGATCCATGCAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)88275 - 362
NM_006157 (NELL1)88275 - 362
Exon 3 To Exon 4 (Pair 10)
Forward (366,21)TTGGAGTTGCTCAGGTGTCTGAmplicon SizecDNA: 82 bpgenomic: 105,701
Reverse (447,22)GCTGCATGGATCTCTCTTTCTANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.232 kcal/mol in 3' area
Forward Dimers-0.293 kcal/mol
Reverse Loops2.093 kcal/mol
Reverse Dimers-1.072 kcal/mol
Forward-Reverse Dimers-1.031 kcal/mol
Amplicon Folding-0.483 kcal/mol outside primersite
Amplicon SequenceTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACA
TAGAAAGAGAGATCCATGCAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)82281 - 362
NM_006157 (NELL1)82281 - 362
Exon 4 To Exon 5 (Pair 1)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 92 bpgenomic: 63,844
Reverse (627,23)ATGTAGTGATACCGAATCTCATCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACAT
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)92451 - 542
NM_006157 (NELL1)92451 - 542
Exon 4 To Exon 5 (Pair 2)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 99 bpgenomic: 63,851
Reverse (631,23)GTGTATGTAGTGATACCGAATCTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)99448 - 546
NM_006157 (NELL1)99448 - 546
Exon 4 To Exon 5 (Pair 3)
Forward (548,21)CTGTCCATTCGAGAACTGGAGAmplicon SizecDNA: 182 bpgenomic: 63,934
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.164 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceCTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGAT
GAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGC
ATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTC
CA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)182463 - 644
NM_006157 (NELL1)182463 - 644
Exon 4 To Exon 5 (Pair 4)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 105 bpgenomic: 63,857
Reverse (649,21)TGTCCTTGGCTTCCCATTGTGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)105460 - 564
NM_006157 (NELL1)105460 - 564
Exon 4 To Exon 5 (Pair 5)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 108 bpgenomic: 63,860
Reverse (640,23)CTTCCCATTGTGTATGTAGTGATNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)108448 - 555
NM_006157 (NELL1)108448 - 555
Exon 4 To Exon 5 (Pair 6)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 107 bpgenomic: 63,859
Reverse (640,23)CTTCCCATTGTGTATGTAGTGATNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)107449 - 555
NM_006157 (NELL1)107449 - 555
Exon 4 To Exon 5 (Pair 7)
Forward (553,21)CATTCGAGAACTGGAGCACAGAmplicon SizecDNA: 177 bpgenomic: 63,929
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.586 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.149 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAGAT
TCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGCATGGC
AGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)177468 - 644
NM_006157 (NELL1)177468 - 644
Exon 4 To Exon 5 (Pair 8)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 95 bpgenomic: 63,847
Reverse (627,23)ATGTAGTGATACCGAATCTCATCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACAT
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)95448 - 542
NM_006157 (NELL1)95448 - 542
Exon 4 To Exon 5 (Pair 9)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 105 bpgenomic: 63,857
Reverse (640,23)CTTCCCATTGTGTATGTAGTGATNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)105451 - 555
NM_006157 (NELL1)105451 - 555
Exon 4 To Exon 5 (Pair 10)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 105 bpgenomic: 63,857
Reverse (648,21)GTCCTTGGCTTCCCATTGTGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)105459 - 563
NM_006157 (NELL1)105459 - 563
Exon 4 To Exon 5 (Pair 11)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 92 bpgenomic: 63,844
Reverse (624,22)TAGTGATACCGAATCTCATCCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.435 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)92448 - 539
NM_006157 (NELL1)92448 - 539
Exon 4 To Exon 5 (Pair 12)
Forward (550,21)GTCCATTCGAGAACTGGAGCAAmplicon SizecDNA: 180 bpgenomic: 63,932
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.164 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGA
GATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGCAT
GGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)180465 - 644
NM_006157 (NELL1)180465 - 644
Exon 4 To Exon 5 (Pair 13)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 112 bpgenomic: 63,864
Reverse (645,22)CTTGGCTTCCCATTGTGTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)112449 - 560
NM_006157 (NELL1)112449 - 560
Exon 4 To Exon 5 (Pair 14)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 96 bpgenomic: 63,848
Reverse (629,23)GTATGTAGTGATACCGAATCTCANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)96449 - 544
NM_006157 (NELL1)96449 - 544
Exon 4 To Exon 5 (Pair 15)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 100 bpgenomic: 63,852
Reverse (643,22)TGGCTTCCCATTGTGTATGTAGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops0.968 kcal/mol
Reverse Dimers-0.205 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)100459 - 558
NM_006157 (NELL1)100459 - 558
Exon 4 To Exon 5 (Pair 16)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 89 bpgenomic: 63,841
Reverse (624,22)TAGTGATACCGAATCTCATCCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.435 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)89451 - 539
NM_006157 (NELL1)89451 - 539
Exon 4 To Exon 5 (Pair 17)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 102 bpgenomic: 63,854
Reverse (645,22)CTTGGCTTCCCATTGTGTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)102459 - 560
NM_006157 (NELL1)102459 - 560
Exon 4 To Exon 5 (Pair 18)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 106 bpgenomic: 63,858
Reverse (649,21)TGTCCTTGGCTTCCCATTGTGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)106459 - 564
NM_006157 (NELL1)106459 - 564
Exon 4 To Exon 5 (Pair 19)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 91 bpgenomic: 63,843
Reverse (624,22)TAGTGATACCGAATCTCATCCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.435 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)91449 - 539
NM_006157 (NELL1)91449 - 539
Exon 4 To Exon 5 (Pair 20)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 98 bpgenomic: 63,850
Reverse (642,22)GGCTTCCCATTGTGTATGTAGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.539 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)98460 - 557
NM_006157 (NELL1)98460 - 557
Exon 4 To Exon 5 (Pair 21)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 99 bpgenomic: 63,851
Reverse (643,22)TGGCTTCCCATTGTGTATGTAGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops0.968 kcal/mol
Reverse Dimers-0.205 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)99460 - 558
NM_006157 (NELL1)99460 - 558
Exon 4 To Exon 5 (Pair 22)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 103 bpgenomic: 63,855
Reverse (647,22)TCCTTGGCTTCCCATTGTGTATNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)103460 - 562
NM_006157 (NELL1)103460 - 562
Exon 4 To Exon 5 (Pair 23)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 111 bpgenomic: 63,863
Reverse (643,22)TGGCTTCCCATTGTGTATGTAGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.968 kcal/mol
Reverse Dimers-0.205 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)111448 - 558
NM_006157 (NELL1)111448 - 558
Exon 4 To Exon 5 (Pair 24)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 96 bpgenomic: 63,848
Reverse (631,23)GTGTATGTAGTGATACCGAATCTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)96451 - 546
NM_006157 (NELL1)96451 - 546
Exon 4 To Exon 5 (Pair 25)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 99 bpgenomic: 63,851
Reverse (642,22)GGCTTCCCATTGTGTATGTAGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops1.539 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)99459 - 557
NM_006157 (NELL1)99459 - 557
Exon 4 To Exon 5 (Pair 26)
Forward (551,21)TCCATTCGAGAACTGGAGCACAmplicon SizecDNA: 179 bpgenomic: 63,931
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.164 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAG
ATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGCATG
GCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)179466 - 644
NM_006157 (NELL1)179466 - 644
Exon 4 To Exon 5 (Pair 27)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 113 bpgenomic: 63,865
Reverse (645,22)CTTGGCTTCCCATTGTGTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)113448 - 560
NM_006157 (NELL1)113448 - 560
Exon 4 To Exon 5 (Pair 28)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 106 bpgenomic: 63,858
Reverse (641,22)GCTTCCCATTGTGTATGTAGTGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)106451 - 556
NM_006157 (NELL1)106451 - 556
Exon 4 To Exon 5 (Pair 29)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 109 bpgenomic: 63,861
Reverse (642,22)GGCTTCCCATTGTGTATGTAGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.539 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)109449 - 557
NM_006157 (NELL1)109449 - 557
Exon 4 To Exon 5 (Pair 30)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 185 bpgenomic: 63,937
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTAC
CGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTG
CTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)185460 - 644
NM_006157 (NELL1)185460 - 644
Exon 4 To Exon 5 (Pair 31)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 110 bpgenomic: 63,862
Reverse (645,22)CTTGGCTTCCCATTGTGTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)110451 - 560
NM_006157 (NELL1)110451 - 560
Exon 4 To Exon 5 (Pair 32)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 92 bpgenomic: 63,844
Reverse (625,22)GTAGTGATACCGAATCTCATCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.17 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)92449 - 540
NM_006157 (NELL1)92449 - 540
Exon 4 To Exon 5 (Pair 33)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 110 bpgenomic: 63,862
Reverse (642,22)GGCTTCCCATTGTGTATGTAGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.539 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)110448 - 557
NM_006157 (NELL1)110448 - 557
Exon 4 To Exon 5 (Pair 34)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 90 bpgenomic: 63,842
Reverse (625,22)GTAGTGATACCGAATCTCATCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.17 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)90451 - 540
NM_006157 (NELL1)90451 - 540
Exon 4 To Exon 5 (Pair 35)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 97 bpgenomic: 63,849
Reverse (629,23)GTATGTAGTGATACCGAATCTCANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)97448 - 544
NM_006157 (NELL1)97448 - 544
Exon 4 To Exon 5 (Pair 36)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 110 bpgenomic: 63,862
Reverse (643,22)TGGCTTCCCATTGTGTATGTAGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.968 kcal/mol
Reverse Dimers-0.205 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)110449 - 558
NM_006157 (NELL1)110449 - 558
Exon 4 To Exon 5 (Pair 37)
Forward (546,22)TACTGTCCATTCGAGAACTGGAAmplicon SizecDNA: 184 bpgenomic: 63,936
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.164 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceTACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGG
ATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACC
GCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGC
TCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)184461 - 644
NM_006157 (NELL1)184461 - 644
Exon 4 To Exon 5 (Pair 38)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 98 bpgenomic: 63,850
Reverse (630,23)TGTATGTAGTGATACCGAATCTCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)98448 - 545
NM_006157 (NELL1)98448 - 545
Exon 4 To Exon 5 (Pair 39)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 186 bpgenomic: 63,938
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTA
CCGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCT
GCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)186459 - 644
NM_006157 (NELL1)186459 - 644
Exon 4 To Exon 5 (Pair 40)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 107 bpgenomic: 63,859
Reverse (642,22)GGCTTCCCATTGTGTATGTAGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.539 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)107451 - 557
NM_006157 (NELL1)107451 - 557
Exon 4 To Exon 5 (Pair 41)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 98 bpgenomic: 63,850
Reverse (631,23)GTGTATGTAGTGATACCGAATCTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)98449 - 546
NM_006157 (NELL1)98449 - 546
Exon 4 To Exon 5 (Pair 42)
Forward (552,21)CCATTCGAGAACTGGAGCACAAmplicon SizecDNA: 178 bpgenomic: 63,930
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.469 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAGA
TTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGCATGG
CAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)178467 - 644
NM_006157 (NELL1)178467 - 644
Exon 4 To Exon 5 (Pair 43)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 94 bpgenomic: 63,846
Reverse (629,23)GTATGTAGTGATACCGAATCTCANr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)94451 - 544
NM_006157 (NELL1)94451 - 544
Exon 4 To Exon 5 (Pair 44)
Forward (549,21)TGTCCATTCGAGAACTGGAGCAmplicon SizecDNA: 181 bpgenomic: 63,933
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.164 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGGGATG
AGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCTTACCGCA
TGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTCCTGCTCC
A
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)181464 - 644
NM_006157 (NELL1)181464 - 644
Exon 4 To Exon 5 (Pair 45)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 95 bpgenomic: 63,847
Reverse (630,23)TGTATGTAGTGATACCGAATCTCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)95451 - 545
NM_006157 (NELL1)95451 - 545
Exon 4 To Exon 5 (Pair 46)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 108 bpgenomic: 63,860
Reverse (641,22)GCTTCCCATTGTGTATGTAGTGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)108449 - 556
NM_006157 (NELL1)108449 - 556
Exon 4 To Exon 5 (Pair 47)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 93 bpgenomic: 63,845
Reverse (625,22)GTAGTGATACCGAATCTCATCCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.17 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)93448 - 540
NM_006157 (NELL1)93448 - 540
Exon 4 To Exon 5 (Pair 48)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 104 bpgenomic: 63,856
Reverse (648,21)GTCCTTGGCTTCCCATTGTGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGAC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)104460 - 563
NM_006157 (NELL1)104460 - 563
Exon 4 To Exon 5 (Pair 49)
Forward (533,22)ACTTCAGGAGTGATACTGTCCAAmplicon SizecDNA: 109 bpgenomic: 63,861
Reverse (641,22)GCTTCCCATTGTGTATGTAGTGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops1.902 kcal/mol
Reverse Dimers0.006 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGC
AGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)109448 - 556
NM_006157 (NELL1)109448 - 556
Exon 4 To Exon 5 (Pair 50)
Forward (536,22)TCAGGAGTGATACTGTCCATTCAmplicon SizecDNA: 108 bpgenomic: 63,860
Reverse (643,22)TGGCTTCCCATTGTGTATGTAGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.968 kcal/mol
Reverse Dimers-0.205 kcal/mol
Forward-Reverse Dimers-1.02 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGT
GGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)108451 - 558
NM_006157 (NELL1)108451 - 558
Exon 4 To Exon 5 (Pair 51)
Forward (544,22)GATACTGTCCATTCGAGAACTGAmplicon SizecDNA: 104 bpgenomic: 63,856
Reverse (647,22)TCCTTGGCTTCCCATTGTGTATNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAG
GGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)104459 - 562
NM_006157 (NELL1)104459 - 562
Exon 4 To Exon 5 (Pair 52)
Forward (542,22)GTGATACTGTCCATTCGAGAACAmplicon SizecDNA: 188 bpgenomic: 63,940
Reverse (729,20)TGGAGCAGGAGATGAGAGGCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.996 kcal/mol
Forward Dimers-1.184 kcal/mol
Reverse Loops1.032 kcal/mol
Reverse Dimers-0.584 kcal/mol
Forward-Reverse Dimers-1.36 kcal/mol
Amplicon Folding-0.935 kcal/mol outside primersite
Amplicon SequenceGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTG
AGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAGGACAGAGGCACTTCCT
TACCGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTCTCATCTC
CTGCTCCA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)188457 - 644
NM_006157 (NELL1)188457 - 644
Exon 4 To Exon 5 (Pair 53)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 94 bpgenomic: 63,846
Reverse (627,23)ATGTAGTGATACCGAATCTCATCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACAT
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)94449 - 542
NM_006157 (NELL1)94449 - 542
Exon 4 To Exon 5 (Pair 54)
Forward (534,22)CTTCAGGAGTGATACTGTCCATAmplicon SizecDNA: 97 bpgenomic: 63,849
Reverse (630,23)TGTATGTAGTGATACCGAATCTCNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops0.947 kcal/mol
Forward Dimers-0.889 kcal/mol
Reverse Loops0.9 kcal/mol
Reverse Dimers-0.996 kcal/mol
Forward-Reverse Dimers-1.014 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceCTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCA
GTGGCCTGAGGGATGAGATTCGGTATCACTACATACA
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)97449 - 545
NM_006157 (NELL1)97449 - 545
Exon 4 To Exon 5 (Pair 55)
Forward (545,22)ATACTGTCCATTCGAGAACTGGAmplicon SizecDNA: 101 bpgenomic: 63,853
Reverse (645,22)CTTGGCTTCCCATTGTGTATGTNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops1.975 kcal/mol in 3' area
Forward Dimers-1.184 kcal/mol
Reverse Loops0.897 kcal/mol
Reverse Dimers-0.276 kcal/mol
Forward-Reverse Dimers-1.025 kcal/mol
Amplicon Folding-0.702 kcal/mol outside primersite
Amplicon SequenceATACTGTCCATTCGAGAACTGGAGCACAGCTATTTTGAACTGGAGAGCAGTGGCCTGAGG
GATGAGATTCGGTATCACTACATACACAATGGGAAGCCAAG
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)101460 - 560
NM_006157 (NELL1)101460 - 560
Exon 5 To Exon 7 (Pair 1)
Forward (710,20)GCCTCTCATCTCCTGCTCCAAmplicon SizecDNA: 159 bpgenomic: 70,490
Reverse (868,22)GATGATCTTCCCATCTTGGATGNr. of Isoforms 2 out of 2 (by amplicon BlastN)
Folding EnergiesForward Loops2.03 kcal/mol
Forward Dimers-0.002 kcal/mol
Reverse Loops0.405 kcal/mol in 3' area
Reverse Dimers-0.905 kcal/mol
Forward-Reverse Dimers-1.458 kcal/mol
Amplicon Folding-0.677 kcal/mol outside primersite
Amplicon SequenceGCCTCTCATCTCCTGCTCCATGTCGACTGTAACAGGATTTATGAGCGTGTGATAGACCCT
CCAGATACCAACCTTCCCCCAGGAATCAATTTATGGCTTGGCCAGCGCAACCAAAAGCAT
GGCTTATTCAAAGGGATCATCCAAGATGGGAAGATCATC
All Amplified targets
 (by primer blast)
TranscriptSizePosition on transcript
NM_201551 (NELL1)159625 - 783
NM_006157 (NELL1)159625 - 783
 

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