Design Results for nmd

(Drosophila melanogaster, BDGP R5 / UCSC dm3)

REMARK: UCSC does not support the FlySNP database. Please check your results for SNPs manually at the FlySNP Project Homepage

Settings Overview
Minimal Amplicon Size70Maximal Amplicon Size300
Natrium Concentration0.3 MMagnesium Concentration0.015 M
Annealing Temperature60 °CMinimal dG for primer folding0
Minimal dG for primer dimer-3 Minimal dG for amplicon folding-3
Nr of bases considered as 3'5Minimal Intron Size3000

The design was not successful. No intron spanning primers were found, see below for details. You can also try to design exon-exon boundary spanning primers here.

Program Output:

Loading ...